This study investigated whether vitamin D receptor gene polymorphism is altered in primary open-angle glaucoma subjects carrying the risk allele and vitamin D deficiency is an important factor in the development of glaucoma. Primary open-angle glaucoma patients and age-matched people in the Han population were enrolled. Serum levels of 1a, 25-Dihydroxyvitamin D3 were measured by enzyme-linked immunoabsorbent assay. Vitamin D receptor polymorphic analysis was studied by real-time polymerase-chain reaction high resolution melting analysis.

Inclusion Criteria:

• intraocular pressure greater than 22 mmHg with two or more medications
• wide anterior chamber angle
• glaucomatous optic neuropathy (Glaucomatous optic nerve damage was defined as cup-to-disc ratio higher than 0.7 or focal loss of the nerve fiber layer (notch) associated with a consistent glaucomatous visual field defect)
• visual field loss consistent with optic nerve damage and visual fields were performed by using standard automated perimetry

Exclusion Criteria:

• the presence of any secondary glaucoma including exfoliation syndrome or a history of ocular trauma
• high myopia
• macular degeneration
• other ocular diseases
• a known history of systemic diseases and administration of vitamin D3 or other analog

primary open-angle glaucoma patients and randomly age-matched people as controls in the Han population of Tianjin China