Protein Intake on Muscle Protein Synthesis Overnight

Effects of Protein Intake on Muscle Protein Synthesis and Whole Body Protein Balance During an Overnight Fast: an Exploratory Study

This trial is an exploratory study in order to determine whether the ingestion of one type of protein (milk protein/CHO/Fat) vs.placebo (CHO/Fat) 2 hours before bed time results in the extension of the post-prandial window of anabolism during the night by increasing whole body protein balance as well as muscle protein fractional synthesis rate (FSR).

Inclusion Criteria:

• Male
• Healthy as determined by the medical questionnaire and the medical visit including blood sampling
• 55-65y of age
• BMI (19.0 - 25.0 kg/ m²)
• Having signed the consent form

Exclusion Criteria:

• Digestive (intestinal, gastric, hepatic or pancreatic), renal or metabolic disease, as determined by the medical (screening) visit and a blood chemistry analysis (glucose, total cholesterol, LDL, HDL, triglycerides, aspartate transaminase, alanine aminotransferase, gamma glutamyl transpeptidase, C Reactive Protein, creatinin)
• Heart or other organ disease
• Arterial hypertension (Systolic pressure ≥ 140 mmHg or diastolic pressure ≥ 90 mmHg)
• Any clotting trouble (diagnosed by Prothrombin time (PT), activated Partial Thromboplastin Time (aPTT), fibrinogen measures and platelets number (the latter is part of a classical blood cells counting at screening)
• Any inflammatory disease in the past four weeks
• Use of specific medications
• Recent major surgery (3 months)
• History of major gastro-intestinal surgery (gastric bypass, intestinal resection etc…)
• History of cancer within the past year
• Significant weight loss during the last 3 months (5% and more)
• Food allergies, especially cow milk protein allergies
• Special diets especially vegetarian, high protein or weight loss program (anamnesis)
• Smokers
• High level structured exercise 3 times a week (e.g.: fitness club, competitions…)
• Have a high alcohol consumption (more than 2 drinks/day)
• Consumption of illicit drugs anamnesis only
• Having given blood within the last month, or willing to make a blood donation until one month following the end of the study
• Subject who cannot be expected to comply with the study procedures, including consuming the test products.
• Currently participating or having participated in another clinical trial during the last month prior to the beginning of this study