Purpose of the Study:This study aims to evaluate the effectiveness of combining Cordyceps supplementation and fartlek training as a recovery and performance enhancement strategy for soccer players. Muscle injuries often occur when athletes train intensely without adequate recovery. Cordyceps contains bioactive compounds with anti-inflammatory and antioxidant properties, while Fartlek training is designed to improve cardiovascular endurance. This study analyses how this combination affects both the physiological recovery and physical performance of soccer players. Research Question: Does the combination of Cordyceps supplementation and Fartlek training effectively reduce muscle damage, decrease oxidative stress, promote bone metabolism, and improve cardiorespiratory fitness in soccer players? Methodology and Biomarkers: To answer this question, the study evaluates specific physiological indicators from the participants' blood samples and physical tests. Muscle damage and recovery are assessed through creatine kinase (CK), interleukin-6 (IL-6), and interleukin-10 (IL-10). Oxidative stress levels are monitored using superoxide dismutase (SOD) and malondialdehyde (MDA). Bone metabolism and health are measured via bone alkaline phosphatase (BALP) and osteocalcin (OST). Additionally, improvements in the athletes' aerobic capacity and overall physical performance are determined by measuring their maximal oxygen consumption (VOâ‚‚max).
Kriteria Inklusi:
Kriteria Eksklusi:
In addition to the participants and outcome assessors, the biostatistician responsible for the final statistical data analysis was also kept completely anonymous in this trial. All datasets were provided to data analysts using pre-coded group labels (Fartlek cordyceps and Fartlek Placebo) instead of the actual intervention names. The independent researcher who generated the randomization allocation securely holds the master code, and it will not be leaked or disclosed to data analysts until all statistical calculations and interpretation of conclusions are formally completed.
The dietary supplement intervention consists of oral administration of Cordyceps sinensis extract. Participants in the experimental group (Fartlek-Cordyceps) are required to ingest a daily dosage of 500 mg Cordyceps sinensis per capsule. The dosing protocol is strictly timed to maximize absorption and recovery: one capsule is taken every night before bed, and on training days, an additional capsule is administered exactly 1 hour prior to the Fartlek session. This supplementation runs concurrently with the physical training period. To maintain the double-blind design, capsules are identical to the placebo in appearance and taste. This targeted timing aims to protect muscle fibers from acute training stress during exercise and accelerate cellular repair overnight, ultimately acting to suppress inflammatory responses, mitigate oxidative damage, and support bone metabolism markers under high physiological loads.
Participants assigned to this comparator arm undergo a structured Fartlek training program while receiving a placebo intervention to maintain the trial's double-blind design. The physical training protocol consists of continuous running sessions with alternating intensity bursts, identical in frequency, duration, and workload to the experimental group. For the supplementation control, participants are required to orally ingest a daily placebo capsule that is visually identical to the active treatment, matched exactly for size, shape, color, and taste. The dosing schedule mimics the experimental protocol: one capsule is taken every night before bed, and on training days, one capsule is administered 1 hour prior to the Fartlek session. This arm serves to isolate the physiological adaptations of the physical training alone and controls for the placebo effect on muscle damage, inflammation, oxidative stress, bone metabolism, and VO2max.
Surabaya, Jawa Timur - East Java 60213, Indonesia
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