The Aim of the Study is to Check Whether Follicular Fluid Evaluation Using a Spectrophotometer Can Differentiate High Quality Oocytes From Low Quality Oocytes. Oocyte Quality Will be Defined Using GDF9, BMP15 and Oocyte Morphology.

Oocyte Quality Assessment Using Spectrophotometer

The aim of the study is to check whether follicular fluid evaluation using a spectrophotometer can differentiate high quality oocytes from low quality oocytes. Oocyte quality will be defined using GDF9, BMP15 and oocyte morphology. The correlation between cortisol levels in women and oocyte quality parameters will be estimated.

Inclusion Criteria:

• Women aged 20-40 years.
• Undergoing IVF or IVF-ICSI treatment.
• At least five follicles visualized on ultrasound at the time of oocyte retrieval
• Ability to provide written informed consent.

Exclusion Criteria:

• Age >40 years.
• Fewer than five follicles at the time of oocyte retrieval.
• Use of donor oocytes.
• Significant medical comorbidities affecting ovarian function.
• Known ovarian malignancy or prior ovarian surgery significantly affecting follicular integrity.