Networks of Bacterium-Metabolite Interactions in the Small Intestine

Networks of Bacterium-Metabolite Interactions in the Small Intestine

Probands with an Ileo- or Colostomy are assigned to consume a test meal which is either high in fat or high in carbohydrates. After the test meal samples from the stoma, urine, blood and skin are retrieved. These will be subjected to large scale analyses of microbiota and metabolite content. To get a better comparability test subjects will consume a standardized liquid diet three days before the study day.

Between both study days a two week wash out phase will be done. During the study day sampling will be done at set time points until 8 hours after the consumption of the test meal.

Inclusion Criteria:

• Written informed consent
• General good health
• Ileostomy or colostomy

Exclusion Criteria:

• Hematologic disorders with contraindication of blood draw
• Participation in other clinical trial interfering with study protocol