Ex Vivo Study of the Mechanism of Action of Active Ingredients on the Intestinal Microbiota

Stool Collection From Healthy Donors for the ex Vivo Study of the Mechanism of Action of Active Ingredients on the Intestinal Microbiota: Bacteria / Host Interaction Model

To design and understand the mechanism of action of different combinations of nutraceuticals coupling bacteria, fibers and polyphenols, which can act on the 4 pillars simultaneously via an innovative ex-vivo model approach coupled with functional and quantitative metagenomics.

The objective of the study is to collect fresh stools from 4 healthy volunteers, in order to study the mechanism of action of probiotic strains, polyphenols and fibers, alone or in combinations. These fresh stools will be cultured in vitro in fermenters, and the samples taken at different culture times will be analyzed in terms of microbial composition (shotgun metagenomic analysis) and their cellular activity (functional metagenomic analysis).

Inclusion Criteria:

• Person with a low fiber diet (<17g / day).

Exclusion Criteria:

• Taking antibiotics and / or transit modulators in the 3 months preceding the sample;
• Colonoscopy in the last 3 months;
• Person under legal protection measure (curators, tutors, etc.).