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Halted after intervention group completed. Control group with only 3 participants
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The investigators will recruit probands with a ileo- or colostoma that are otherwise in good general health and analyse the stomal fluids for their microbiota composition. Sampling will be performed over a time span of 28 days, after 14 days probands will modify their base-line diet to a low carbohydrate diet.
Additionally to stoma fluids which will be analyzed for their microbiota composition the investigators will also collect skin samples to analyze for microbiota composition and urine to analyze for metabolite composition.
Low carbohydrate diet will allow for consumption of 80g complex carbohydrates per day, simple sugars will have to be avoided completely
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| All study participants | Experimental | All participants will undergo sample collection during their regular diet and after modification of their diet to a low carbohydrate diet |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Diet modification | Other | Participants will reduce their carbohydrate consumption to 80g daily |
|
| Measure | Description | Time Frame |
|---|---|---|
| Assessment of microbiota profile by 16S sequencing before and after diet modification for significant differences | Sample content will be measured by 16S sequencing, ecological distances will be assessed by beta-diversity | 28 days |
| Measure | Description | Time Frame |
|---|---|---|
| Relative changes of total metabolite abundance in urine | The whole metabolite composition will be assessed by an untargeted screen done with mass spectrometry | 28 days |
| Analysis of microbiota metabolic potential (microbiome DNA coding for enzymes) after shotgun sequencing |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Benjamin Misselwitz, Prof. Dr. med. | University Clinic for Visceral Surgery and Medicine | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Inselspital | Bern | 3010 | Switzerland |
Sharing of raw sequencing data as electronic supplement to publication (e.g. by entry on database)
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upon publication, unlimited
Open access
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| ID | Term |
|---|---|
| D004035 | Diet Therapy |
| ID | Term |
|---|---|
| D044623 | Nutrition Therapy |
| D013812 | Therapeutics |
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First phase will be purely observational, second phase involves diet modification
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The total DNA content of the samples will be annotated for for known metabolic active enzymes to assess functionality |
| 28 days |
| Analysis of microbiota biomass | The samples will be analysed for the number of bacteria present in them | 28 days |
| Changes of further metagenomic microbiome composition (viral, eucaryotic DNA) | The DNA of the samples will be screened for parts belonging to viruses or eucaryotic cells | 28 days |
| Culturing of microbiota if appropriate (in vitro) | If of interest, culturing of specific bacterial strains will be attempted in vitro with the aim to recreate in vivo observations | 28 days |