Measurement of vanilloïd Compounds in Urin of Autistic vs Control Patients, as Potential Biomarkers

Diagnostic of Autism by Measurement of vanilloïd Compounds in Urin

In a former study, four vanilloid compounds were observed increased in the urin of autistic patients. The present study is aimed at performing a ambispective study to confirm or not these preliminary results. 70 urins from autistic children (already collected) will be compared to 70 urins of non autistic children (collected without intervention, remaining sample from the standard care), in terms of measurements of four vanilloid compounds using a GCMS apparatus.

Inclusion criteria :

For cases :

• Male children aged 2 years to less than 15 years inclusive years at time of sample collection
• Children with a diagnosis of autism (ICD-10 F84.0) after assessment by a child psychiatrist.

For healthy chlidren:

• Children aged 2 years to less than 15 years old performing an ECBU at the CHRU of Nancy as part of their care.
• Patient whose ECBU is negative with respect to cytobacteriology (absence of urinary infection).
• Child can be matched to a CAS

Exclusion criteria

For cases:

• Children in whom one of the following pathologies is found during the anamnesis or in the clinical record : (i) renal insufficiency; (ii) urinary tract infection at the time of sampling and/or metabolic disease
• Children on antibiotic treatment at the time of collection

For healthy chlidren :

• children whose urine culture come from one of the following departments: Pediatric Nephrology (potential risk of renal failure) or Pediatric Neurology (potential risk of neurological disorder).
• with a diagnosis of autism
• with a metabolic disease
• children undergoing antibiotic treatment at the time of collectio