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This parallel-arm trial study investigates the effects of restricting arterial blood flow during cycling exercise on citrate synthase. Participants will be randomized to either a blood flow restricted or non-blood flow restricted exercise training group. Both groups will complete seven, 30-minute exercise sessions over a 14-day period.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| BFR | Experimental |
| |
| CTL | Experimental |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| BFR Exercise Training | Other | Participants will complete exercise training on a recumbent bike placed on a 45 degree incline (BFR). |
|
| Measure | Description | Time Frame |
|---|---|---|
| Citrate Synthase | Fold change in citrate synthase maximal activity | 3 days prior to start of exercise training and 3 days after completing exercise training |
| Measure | Description | Time Frame |
|---|---|---|
| Transcription factor A, mitochondrial (TFAM) | Fold change in TFAM protein | 3 days before training to 3 days after training |
| Nuclear respiratory factor, 1 (NRF1) | Fold change in NRF-1 protein |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Brendon J Gurd, PhD | Queen's University | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Queen's University | Kingston | Ontario | K7L3N6 | Canada |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 40224168 | Derived | Preobrazenski N, Mladen SPS, Causer E, Menezes E, Islam H, Drouin PJ, Tschakovsky ME, Gurd BJ. Mitochondrial and cardiovascular responses to aerobic exercise training in supine and upright positions in healthy young adults: a randomized parallel arm trial. Transl Exerc Biomed. 2025 Mar 26;2(1):9-20. doi: 10.1515/teb-2025-0002. eCollection 2025 May. |
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Allocation concealment, assessor blinding
| CTL Exercise Training | Other | Participants will complete exercise training on a recumbent bike placed on flat ground (CTL). |
|
| 3 days before training to 3 days after training |
| Maximal oxygen consumption (VO2peak) | Change in VO2peak (POST-PRE) | 3 days before training to 3 days after training |
| Heart rate (HR) | Average HR | Every 5 minutes during each of the 7, 30-minute exercise sessions |
| Revolutions per minute (RPM) | Average RPM | Every 5 minutes during each of the 7, 30-minute exercise sessions |
| Blood [lactate] | Average blood lactate concentration | During all exercise sessions at 10 and 30 minutes |
| Rating of perceived exertion (RPE) | Average RPE | Every 5 minutes during each of the 7, 30-minute exercise sessions |
| Nuclear Factor, Erythroid 2 Like 2 (NFE2L2) | Fold change in NFE2L2 | 3 days before training to 3 days after training |
| Cytochrome c oxidase (COX) | Fold change in COX | 3 days before training to 3 days after training |
| Electromyography (EMG) | Median frequency and average amplitude of EMG signal | Throughout each of the 7, 30-minute exercise sessions |
| Near-infrared spectroscopy (NIRS) | Deoxygenated hemoglobin and oxygenated hemoglobin | Throughout each of the 7, 30-minute exercise sessions |