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| ID | Type | Description | Link |
|---|---|---|---|
| MOST 111-2410-H-845 -005 -MY3 | Other Grant/Funding Number | National Science and Technology Council |
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In this 3-year project, investigators will first measure stem cells (primary outcome) and immune cells (secondary outcome) in human skeletal muscle immediately after and 24 hour after the BFR-training. In the second and third year, the effect of β-NMN (vitamin B3 precursor) and antioxidant on the same outcomes after BFR-exercise will be assessed. The BFR-exercise in the study will consist of a 5 × 10-second sprinting with maximal voluntary efforts, 5 minutes after a BFR pre-conditioning to legs using pressurized cuffs. In Study 1 (1st year, N = 24, aged 20-30 year), a randomized crossover trial will be conducted to compare the efficacy of sprinting exercise using BFR pre-conditioning at 20 mmHg (control) or 180 mmHg (occlusion). Study 2 (2nd year, N = 24, aged 20-30 years) will be conducted to examine the effect of β-NMN supplementation on the aforementioned outcomes in human muscles using a randomized placebo-controlled crossover study design. Study 3 (third year, N = 24, aged 20-30 years) will be conducted to examine the effect of vitamin C on the same variables immediately after and 24 hours after resistance exercise intervention, using a randomized placebo-controlled crossover study design.
Primary outcome: Stem cells (Stro-1+ cells) assessed by immunofluorescence staining.
Secondary outcome: Immune cells (MPO+ cells) assessed by immunofluorescence staining.
Study 1: Two-arm intervention (counterbalanced crossover design): Sham-exercise, BFR-exercise Study 2: Two-arm intervention (counterbalanced crossover design): Placebo-BFR exercise, NMN-BFR exercise Study 3: Two-arm intervention (counterbalanced crossover design): Placebo-BFR exercise, Vitamin C-BFR exercise
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Sham (20 mmHg) | Sham Comparator | Study 1: Sham: blood flow restriction (20 mmHg on leg) for 3-5 min before exercise |
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| BFR (180 mmHg) | Experimental | Study 1: BFR: blood flow restriction (180 mmHg on leg) for 3-5 min before exercise |
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| Placebo-BFR exercise | Placebo Comparator | Study 2: Placebo (cornstarch)-ingestion before and after BFR-exercise |
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| NMN-BFR exercise | Experimental | Study 2: NMN-ingestion before and after BFR-exercise |
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| Placebo-resistance exercise | Placebo Comparator | Study 3: Cornstarch-ingestion before and after resistance exercise |
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| Vitamin C-resistance exercise | Experimental |
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| BFR (180 mmHg) | Behavioral | Sprinting exercise after BFR |
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| Measure | Description | Time Frame |
|---|---|---|
| Stem cells in muscle | Stem cells are identified by Stro-1 cell marker | Pre-exercise, immediately post-exercise, and 24-hour post-challenge (BFR-exercise) recovery |
| Measure | Description | Time Frame |
|---|---|---|
| Immune cells in muscle | Immune cells are identified by myeloperoxidase (MPO) cell marker | At 24-hour post-challenge recovery |
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Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Laboratory of Exercise Biochemistry | Taipei | Taiwan | 111 | Taiwan |
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| Type | Includes Protocol | Includes SAP | Includes ICF | Document Label | Document Date | Document Uploaded Date | Document File Name |
|---|---|---|---|---|---|---|---|
| Prot | Yes | No | No | Study Protocol | Aug 1, 2022 | May 1, 2026 | Prot_000.pdf |
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| ID | Term |
|---|---|
| D009043 | Motor Activity |
| ID | Term |
|---|---|
| D001519 | Behavior |
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| ID | Term |
|---|---|
| C005703 | salicylhydroxamic acid |
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Three studies will be conducted:
Study 1: 2-arm Study 2: 2-arm Study 3: 2-arm
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Study 3: Vitamin C-ingestion before and after BFR-exercise |
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| NMN-BFR exercise | Dietary Supplement | NMN ingested before and after BFR exercise |
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| Vitamin C-resistance exercise | Dietary Supplement | Vitamin C ingested before and after resistance exercise |
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