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| ID | Type | Description | Link |
|---|---|---|---|
| 402635 | Other Grant/Funding Number | NSERC |
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This crossover study investigates the effects of restricting arterial blood flow during cycling exercise on PGC-1α and its upstream signalling pathways. In a randomized order, participants will exercise once for 30 minutes with restricted blood flow and then will exercise once for 30 minutes with no blood flow restriction.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| CTL - BFR | Experimental | CTL Exercise then BFR Exercise |
|
| BFR - CTL | Experimental | BFR Exercise then CTL Exercise |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| BFR Exercise | Other | Participants will exercise on a recumbent bike placed on a 45 degree incline (BFR). |
|
| Measure | Description | Time Frame |
|---|---|---|
| Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA) | Fold-change in PGC-1α mRNA expression | 15 minutes before exercise and 3 hours post-exercise for both exercise sessions |
| Measure | Description | Time Frame |
|---|---|---|
| Catecholamine concentrations | Catecholamine concentrations in venous blood | 15 minutes before exercise and immediately post-exercise for both exercise sessions |
| Total and phosphorylated p38 mitogen-activated protein kinase (MAPK) |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Brendon J Gurd, PhD | Queen's University | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Queen's University | Kingston | Ontario | K7L3N6 | Canada |
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| Type | Includes Protocol | Includes SAP | Includes ICF | Document Label | Document Date | Document Uploaded Date | Document File Name |
|---|---|---|---|---|---|---|---|
| SAP | No | Yes | No | Statistical Analysis Plan | Jun 24, 2019 | Jun 24, 2019 | SAP_001.pdf |
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| ID | Term |
|---|---|
| D000090003 | Blood Flow Restriction Therapy |
| ID | Term |
|---|---|
| D005081 | Exercise Therapy |
| D012046 | Rehabilitation |
| D000359 | Aftercare |
| D003266 | Continuity of Patient Care |
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Allocation concealment, assessor blinding
| CTL Exercise | Other | Participants will exercise on a recumbent bike placed on flat ground (CTL). |
|
Fold change in p38 MAPK phosphorylation
| 15 minutes before exercise and immediately post-exercise for both exercise sessions |
| Total and phosphorylated acetyl coenzyme A carboxylase (ACC) | Fold change in ACC phosphorylation | 15 minutes before exercise and immediately post-exercise for both exercise sessions |
| Total and phosphorylated protein kinase A (PKA) | Fold change in PKA phosphorylation | 15 minutes before exercise and immediately post-exercise for both exercise sessions |
| Acetylated p53 | Fold change in acetylated p53 | 15 minutes before exercise and immediately post-exercise for both exercise sessions |
| Blood lactate | Average [lactate] between conditions | During both exercise sessions (lactate); 15 minutes before and immediately after both exercise sessions |
| Heart rate (HR) | Average HR between conditions | During both exercise sessions |
| Revolutions per minute (RPM) | Average RPM between conditions | During both exercise sessions |
| Ratings of perceived exertion (RPE) | Average RPE between conditions | During both exercise sessions |
| Oxygenated and deoxygenated hemoglobin | Average oxygenated and deoxygenated hemoglobin as a percentage of same day max values | During both exercise sessions and up to 10 minutes after (for cuff occlusion) |
| D005791 |
| Patient Care |
| D013812 | Therapeutics |
| D026741 | Physical Therapy Modalities |