Data and knowledge gathered on bioavailability of coffee phenolics is becoming more and more important, hence underlying the importance of better understanding the fate of these potential health promoting antioxidants. However, some analytical barriers as well as some key aspects of metabolism still remain to be fully elucidated to get the full picture of coffee metabolism and bioavailability. One aspect addressed in the present study is the impact of food matrix in modulating absorption, plasma appearance and urinary excretion of coffee bioactives.

Inclusion Criteria:

• Healthy men and women
• Aged 20-44 years
• Caucasian origin
• Body mass index (BMI) 19-25 kg/m2
• Used to drinking coffee (similar to study coffee) on a daily basis
• Being able to tolerate a 48-hour coffee abstinence
• Normal oral glucose tolerance test
• Having signed the informed consent form

Exclusion Criteria:

• Taking medication or dietary supplements
• Smoking
• Performing a competitive sport
• Having metabolic disorders
• Long gut transit time (>24 h)
• Blood donor
• Irregularity in menstrual cycle (for women)
• Pregnancy (for women)
• Subject who cannot be expected to comply with the study procedures.
• Currently participating or having participated in another clinical trial during the last 4 weeks prior to the beginning of this study.