This randomized split-mouth clinical trial aims to evaluate the effectiveness of injectable platelet-rich fibrin (i-PRF) in thickening thin gingival phenotype and improving soft tissue conditions around teeth.

Thin gingival tissue is an important risk factor for gingival recession and may negatively affect periodontal health and esthetic outcomes. This study investigates whether the use of i-PRF, obtained from the patient's own blood, can enhance gingival thickness and support tissue healing.

Following initial periodontal therapy, the procedure will be performed under local anesthesia. A minimally invasive approach will be applied to the gingival tissue, and i-PRF prepared from the patient's blood will be injected into the treatment area. The procedure will be repeated in multiple sessions at regular intervals. Patients will receive standard post-operative care and instructions.

Clinical parameters will be evaluated at follow-up visits, and gingival thickness will be measured using ultrasonography over a 6-month period.

Possible risks include mild pain, bleeding, infection, and temporary discomfort, all of which are manageable with routine clinical care.

This study is expected to contribute to the development of minimally invasive and biologically based treatment approaches, reduce the need for additional surgical procedures, and improve periodontal and esthetic outcomes.

Inclusion Criteria:

• Systemically healthy individuals aged ≥18 years
• Non-smokers or light smokers (≤10 cigarettes per day)
• Individuals with full-mouth plaque score (FMPS) <15% and full-mouth bleeding score (FMBS) <15%
• Individuals with gingival thickness <0.8 mm in the mandibular anterior teeth to be treated
• Periodontally healthy individuals with probing depth (PD) ≤3 mm in the mandibular anterior teeth
• Absence of structural defects, crowns, or large restorations in the mandibular anterior teeth
• Absence of missing or supernumerary teeth in the mandibular anterior region
• No history of blood-borne infectious diseases
• No systemic diseases or medical conditions

Exclusion Criteria:

• Individuals under 18 years of age
• Pregnancy or breastfeeding
• Presence of periodontal pockets >4 mm
• Presence of structural defects, crowns, or large restorations in the teeth
• Presence of systemic diseases or coagulation disorders
• Use of anticoagulant medications
• Ongoing orthodontic treatment with active tooth movement
• Use or history of medications affecting periodontal tissues (e.g., immunosuppressive drugs, calcium channel blockers, anticonvulsants)
• History of periodontal surgery in the area to be treated