our study explores the effectiveness of two advanced sperm selection techniques-PICSI and the ZyMot microfluidic device-in improving outcomes of Intracytoplasmic Sperm Injection (ICSI). It aims to compare their impact on fertilization rates, embryo quality, and clinical pregnancy success, helping determine which method offers superior results in assisted reproductive technology.

Inclusion criteria:-

• Age for women is between 20-39 years old.
• Body mass index (BMI) between 19 kg/m and 35 kg/m
• Normal Prolactin level.
• No other endocrinological abnormality including thyroid disorder or diabetes.
• With antimullerian hormone (AMH) at least 1.5 ng/ml, ), with normal progesterone level.
• Primary or secondary infertility at least of 2 years duration.
• No evidence of endometriosis.
• No other medical or surgical disease.
• The day of embryo freezing is the fifth day (day 5), and all patients who undergo the process of transferring frozen embryos.
• The endometrium thickness is between 8-14 mm on day of transfer.
• Inclusion criteria of male partner were; presence of ejaculate motile spermatozoa with total sperm number 1 million/ml at least and sperm motility 5% or more and all patient with high DNA fragmentation index above 35%.

Exclusion criteria׃-

• Women were older than 40 years of age or younger than 18 years old.
• Women laparoscopically diagnosed to have endometriosis within the last 1 year.
• Male infertility factor with immotile sperm.