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Comparing different advanced sperm selection techniques like Physiological ICSI (PICSI) and magnetic activated cell sorting (MACS) in terms of the PGT-A outcomes of each arm blastocysts
Advanced sperm selection methods like PICSI and MACS have been developed for selecting a healthy mature non apoptotic sperm with lower Sperm DNA fragmentation. Previous studies compared the pre-implantation embryo development parameters of those techniques, but none looked at comparing the PGT-A results or euploidy rates of the blastocysts derived from each sperm selection.
PGT-A cases will be randomized on the day of ICSI to the 2 assigned sperm selection techniques.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| PICSI | Active Comparator | Semen processing is done by double layer density gradient method followed by adding Sperm to the dot of hyaluronan on the PICSI dish, within minutes the bound sperm are attached by their acrosome to the surface of the dot. Individual bound sperm selection is done followed y ICSI |
|
| MACS | Active Comparator | Semen processing is done by double layer density gradient method. The resulted pellet is labeled with annexin V microbeads followed by separation on MACS Column, the eluted fraction contains non apoptotic sperm suitable for ICSI. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| PICSI | Other | Sperm selection using PICSI dish for selecting sperm with lower DNA fragmentation index |
|
| Measure | Description | Time Frame |
|---|---|---|
| Euploidy rate | Defined as the proportion of euploid blastocysts | 15 days post ICSI |
| Aneuploidy rate | Defined as the proportion of aneuploid blastocysts | 15 days post ICSI |
| Low mosaic rate | Defined as the proportion of low mosaic blastocysts | 15 days post ICSI |
| High mosaic rate | Defined as the proportion of high mosaic blastocysts | 15 days post ICSI |
| Measure | Description | Time Frame |
|---|---|---|
| Fertilization rate | Defined as the proportion of fertilizaed oocytes | 1 day |
| Cleavage rate | Defined as the proportion of cleaved embryos on day 3 |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Eman Hasanen, BSc. | Ganin Fertility Center | Principal Investigator |
| Manar Hozayen, MSc. | Ganin Fertility Center | Principal Investigator |
| Amr Elshimy, BSc. | Ganin Fertility Center | Principal Investigator |
| Hanaa Alkhader, MBBS/MD | Ganin Fertility Center | Principal Investigator |
| Hosam Zaki, MSc, FRCOG | Ganin Fertility Center | Study Director |
| Yara Hazem, BP | Ganin Fertility Center | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Ganin Fertility Center | Cairo | Maadi | 11728 | Egypt |
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| ID | Term |
|---|---|
| D007246 | Infertility |
| ID | Term |
|---|---|
| D000091662 | Genital Diseases |
| D000091642 | Urogenital Diseases |
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| MACS | Other | Sperm selection using MACS for selecting sperm with lower DNA fragmentation index |
|
| 3 days |
| Blastocyst development rate | Defined as the proportion of blastocysts formed on day 5 or 6 | 5-6 days |
| Blastocyst quality rate | Defined as the assessment of blastocyst quality according to Gardner's criteria into: good, fair or poor | 5-6 days |