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| Name | Class |
|---|---|
| Lesaffre International | INDUSTRY |
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Given the relatively high carbon footprint and sustainability of animal-based proteins, there is a growing interest in determining the nutritional quality non-animal-based protein sources. The overall objective of this investigation is to examine the impact of different animal and microbial-based protein sources to support whole body protein synthesis in adults. To do this, investigators will employ a 'breath test' method developed in our laboratory as well as urine sampling. The results of this study will allow us to better understand the impact of dietary protein quality for maintaining health and body protein mass in adults.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Yeast Protein Supplement | Experimental | Participants will be consuming test beverage composed of yeast protein and their whole-body protein metabolism will be determined over the subsequent 8 hours. |
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| Whey Protein Isolate Supplement | Active Comparator | Participants will be consuming test beverage composed of whey protein isolate and their whole-body protein metabolism will be determined over the subsequent 8 hours. |
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| Collagen Hydrolysate Supplement | Active Comparator | Participants will be consuming test beverage composed of collagen protein and their whole-body protein metabolism will be determined over the subsequent 8 hours. |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Yeast Protein Supplement | Dietary Supplement | Each protein will be consumed at 0.9g/kg/d in n=8 hourly drinks |
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| Measure | Description | Time Frame |
|---|---|---|
| 1. Phenylalanine excretion (umol/kg/h) | Whole body phenylalanine excretion determined from the product of breath 13CO2 enrichment and carbon dioxide production rate (VCO2). | 8 hours |
| Phenylalanine oxidation (umol/kg/h) | Whole-body phenylalanine oxidation determined from the correction of phenylalanine excretion by the phenylalanine precursor enrichment determined from urine enrichment over the 8 hour measurement period. | 8 hours |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Daniel R. Moore, Ph.D. | Contact | 416-946-4088 | drmoore@utoronto.ca |
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Kinesiology & Physical Education | Recruiting | Toronto | Ontario | M5S 2C9 | Canada |
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Single-blind randomized, counterbalanced design
Thirteen healthy young (18-45 years of age, at least 6 females and 6 males) will be subjected to a non-invasive 13CO2 breath-test based on the indicator amino acid oxidation method and urine collections following hourly ingestion of protein test beverages (whey, yeast, and collagen randomized by trials).
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| Whey Protein Isolate Supplement | Dietary Supplement | Each protein will be consumed at 0.9g/kg/d in n=8 hourly drinks |
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| Collagen Hydrolysate Supplement | Dietary Supplement | Each protein will be consumed at 0.9g/kg/d in n=8 hourly drinks |
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