patients will recieve canal disinfection followed by induction of bleeding and placement of platelet rich fibrin as the only scaffold before coronal sealing.

platelet rich fibrin is obtained br removing the middle layer from a centrifuged blood sample and this is rich in platelets that play role in promoting tissue regeneration.

patients will recieve canal disinfection followed by induction of bleeding and injection of this scaffold directly before coronal sealing.

Mesoporous Silica Nanoparticles are biocompatible with high surface area that used as scaffold for enhancing the outcome.

patients will recieve canal disinfection followed by induction of bleeding and injection of this scaffold directly before coronal sealing.

Hyalornic Acid nanoparticles are biodegradable with high water retention capacity promoting pulp tissue regeneration.

patients will recieve canal disinfection followed by induction of bleeding and injection of this scaffold directly before coronal sealing.

Pomegranate Extract Nanoparticles are synthesized from Punica granatum exract with potential antimicrobial and regenerative properties.

platelet rich fibrin is obtained by removing the middle layer from a centrifuged blood sample and is rich with platelets that plays an important role in tissue regeneration mesoporous silica nanoparticles are high surface area and excellent biocompatibility and enhance the outcome hyalurnic acid nanoparticles are high water capacity and involved in cell proliferation and adhesion and help in tissue regeneration pomegranate nanoparticles has anti-inflammatory and antibacterial effect and also help in tissue proliferation and regeneration

radiographic evaluation of apical closure as reduction or elimination of the apical opening of the root