This randomized controlled trial is designed to assess the use of platelet-rich fibrin (PRF), a biomaterial derived from a participant's own blood, in alveolar ridge preservation following premolar extraction for orthodontic purposes. Participants aged 17 to 38 years requiring premolar extractions will be randomly assigned to receive either PRF application or natural healing without intervention. Cone beam computed tomography (CBCT) will be used to evaluate changes in alveolar bone dimensions and density at 30 and 120 days post-extraction. Measurements will be taken at standardized vertical levels (1 mm, 3 mm, and 5 mm apical to the crest) to analyze bone height, width, and density over time.

This study is a randomized controlled clinical trial designed to investigate the application of platelet-rich fibrin (PRF) in post-extraction sites following orthodontically indicated premolar removals. PRF is prepared from autologous blood and applied to extraction sockets in the experimental group, while the control group undergoes standard physiological healing. Cone beam computed tomography (CBCT) will be used to obtain quantitative data on alveolar bone structure at baseline, 30 days, and 120 days post-extraction. The study will assess changes in bone height, horizontal ridge width at defined depths, and bone tissue density using standardized imaging protocols. This design allows for objective comparison between treated and untreated sites.

Inclusion Criteria:

• Healthy individuals with no systemic diseases.
• Age range: 17 to 38 years old.
• Orthodontic indication for premolar extraction (two maxillary or two mandibular premolars).
• Candidates for orthognathic surgery or extraction of impacted/retained teeth (e.g., third molars with a relationship to critical anatomical structures).
• Clinical justification for CBCT imaging instead of standard radiographic -techniques.

Exclusion Criteria:

• Patients with systemic diseases that may alter periodontal therapy outcomes.
• Platelet count below 200,000 mm³.
• Immunosuppressed patients.
• Pregnant or lactating women.
• Smokers.
• Patients taking medications that interfere with healing (e.g., bisphosphonates, corticosteroids, anticoagulants).

Radiographic CBCT images (de-identified) Clinical measurements (bone height, depth, and quality) Demographic information (age, sex, general health status) Outcome measures (PRF effectiveness in alveolar preservation)

Access Criteria:

Available to qualified researchers upon request Requires a formal data access request and institutional agreement Compliance with ethical and privacy regulations

Time Frame:

Available upon study publication Accessible for at least five years post-publication

Storage:

Data will be stored in a secure university repository and potentially in public research databases.

This plan ensures transparency, collaboration, and compliance with ICMJE guidelines for clinical research.

Start Date: Upon publication of study results End Date: At least five years post-publication

Additional Notes:

Data will be made available as soon as the primary results are published in a peer-reviewed journal.

Access may be extended beyond five years based on research demand and institutional policies.

Researchers requesting access must submit a formal data request and adhere to data-sharing agreements and ethical guidelines.

Qualified researchers from academic, research, or healthcare institutions. Investigators conducting meta-analyses or related studies on PRF and alveolar preservation.

Researchers with ethical approval and a valid research proposal.

Available Data:

De-identified CBCT images, clinical measurements (bone height, depth, HU values), demographic data (age, sex, health status), and outcome measures.

Study protocol, data collection methods, and statistical analysis plan.

Access Process:

Submit a formal request detailing research objectives and ethics approval. Sign a data-sharing agreement to ensure ethical use and compliance. Secure data transfer via an encrypted institutional repository. This policy promotes research transparency, collaboration, and ethical data use while protecting participant privacy.