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The influence of a 6000m ergometer test on the markers of gut injury, endotoxemia, iron status, stress and inflammation in elite rowers.
The principal objective of our study was to assess gut permeability after completing a 6000-meter rowing test. The study was performed at the beginning of a competitive training phase. Eighteen elite Polish Rowing Team rowers participated in the study after applying the inclusion/exclusion criteria. The participants performed a 6000-meter ergometer test. Blood samples were taken before the test, after exercise, and after 1 hour of restitution. Parameters, such as I-FABP (intestinal fatty acid binding protein), LPS (lipopolysaccharide), LBP (lipopolysaccharide binding protein), claudin3, ferritin, transferrin, hepcidin, IL-6 (Interleukin-6), HO-1 (Heme Oxygenase 1), and ACSL4 (Long-chain-fatty-acid-CoA ligase 4 ) were determined using appropriate biochemical tests.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| elite rowers |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| exercise test | Other | Participant perforemed 6000m ergometer test. |
|
| Measure | Description | Time Frame |
|---|---|---|
| I-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury | I-FABP concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays | Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period |
| LBP (lipopolysaccharide binding protein) to measure endotoxin | LBP concentration measured in blood [ug/ml] using commercially available enzyme-linked immunosorbent assays | Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period |
| Claudin 3 to measure tight junction leakage | claudin 3 concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays | Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period |
| LPS (lipopolysaccharide) to measure endotoxin | LPS concentration measured in blood [EU/Ll] using commercially available enzyme-linked immunosorbent assays | Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period |
| Lactic acid to measure fatigue after the race | Lactate concentration measured in capillary blood from earlobe [mmol/L] using a commercially available kit (Diaglobal, Berlin, Germany) | Blood samples was taken from earlobe at two-time points: before each stress test (after overnight fasting), one minute after the end of the test. |
| Measure | Description | Time Frame |
|---|---|---|
| energy | energy [kcal ]of food intake measured by 24h dietary recall | day before test, in the morning before the test |
| protein | protein [g ]of food intake measured by 24h dietary recall |
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Inclusion Criteria:
Exclusion Criteria:
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Male rowers age 18 - 24 years.
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Poznan University of Physical Education | Gorzów Wielkopolski | Lubusz Voivodeship | 66-400 | Poland |
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| ID | Term |
|---|---|
| D005080 | Exercise Test |
| ID | Term |
|---|---|
| D006334 | Heart Function Tests |
| D003935 | Diagnostic Techniques, Cardiovascular |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
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| Ferritin to measure iron status | Ferritin concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays | Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period. |
| Transferrin to measure iron status | Transferrin concentration measured in blood [mg/ml] using commercially available enzyme-linked immunosorbent assays | Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period. |
| Hepcidin to measure iron status | Hepcidin concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays | Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period. |
| IL-6 (Interleukin-6) to measure inflammation | IL-6 concentration measured in blood [ng/l] using commercially available enzyme-linked immunosorbent assays. | Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period. |
| HO-1 (Heme Oxygenase 1) to measure oxidative stress | HO-1 concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays | Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period. |
| ACSL4 (Long-chain-fatty-acid-CoA ligase 4 ) to measure oxidative stress | ACSL4 concentration measured in blood [pg/ml] using commercially available enzyme-linked immunosorbent assays. | Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period. |
| day before test, in the morning before the test |
| carbohydrate | carbohydrate [g ]of food intake measured by 24h dietary recall | day before test, in the morning before the test |
| fat | fat [g ]of food intake measured by 24h dietary recall | day before test, in the morning before the test |
| fiber | fiber [g ]of food intake measured by 24h dietary recall | day before test, in the morning before the test |
| body mass | Body mass [kg] measured by electronic scale to the nearest 0,05 kg (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan). | in the morning before the test |
| Body composition - water | % of body water measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan). | in the morning before the test |
| Body composition - fat | % of body fat measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan). | in the morning before the test |
| Lean body mass | Lean body mass [kg] measured by electronic scale to the nearest 0,05 kg (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan). | in the morning before the test |
| Height | Prior to the exercise test, we measured the anthropometric parameters, including height (Seca 213 Hamburg, Deutschland) [cm] | in the morning before the test |
| Gastrointestinal sypthomps self reported scale | 10 point scale were 10 is a outcome of the worst severity | up to 1 hours after the test |
| D012129 | Respiratory Function Tests |
| D003948 | Diagnostic Techniques, Respiratory System |
| D016552 | Ergometry |
| D008919 | Investigative Techniques |