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This study aims to learn about relative energy deficiency in male and female rowers at the end of the direct sport preparation phase of the annual training cycle. The main questions:
- How will energy intake influence leptin, cortisol levels, mood, gut discomfort, and permeability? The observational study involves male and female rowers from the Polish rowing team.
Participants will perform 2000 meter ergometer test and 6000 ergometer test in annual training cycle. Researchers will compare outcomes from both tests.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| male and female rowers | Rowers will perform 2000m ergometer test.A 2,000-m time trial is a standard test used to assess performance in rowers. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| 2000 meter ergometer test | Other | Rowers will perform 2000m ergometer test. A 2,000-meter trial is a standard test used to assess performance in rowers. |
|
| Measure | Description | Time Frame |
|---|---|---|
| -FABP (intestinal fatty acid binding protein)to measure epithelial wall injury | I-FABP concentration measured in blood [ng/ml] using commercially availableenzyme-linked immunosorbent assays | Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 3-hour recovery period |
| Leptin to measure long-term energy balance | Leptin concentration measured in blood [ng/ml] using commercially availableenzyme-linked immunosorbent assays | Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 3-hour recovery period |
| Cortisol to measure stress | Stress hormone concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays | Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 3-hour recovery period |
| sCD14 multifunctional lipopolysaccharide receptor | sCD14 concentration measured in blood [ug/ml] using commercially available enzyme-linked immunosorbent assays | Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 3-hour recovery period |
| Claudin to measure tight junction leakage | Claudin concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays | Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 3-hour recovery period |
| Measure | Description | Time Frame |
|---|---|---|
| energy | energy [kcal ]of food intake measured by food diary | Time Frame: whole day (24hours) before test, in the morning before the test |
| protein | protein [g ]of food intake measured by food diary |
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Inclusion Criteria:
Exclusion Criteria:
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Male rowers age 18 - 22 years.
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Hanna Dziewiecka, Msc | Contact | 606880282 | 48 | hannadziewiecka@gmail.com |
| Anna Skarpańska-Stejnborn, Profesor | Contact |
| Name | Affiliation | Role |
|---|---|---|
| Anna Skarpańska-Stejnborn, Profesor | Poznan University of Physical Education | Study Director |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Poznan University of Physical Education, Faculty of Sport Sciences in Gorzow | Gorzów Wielkopolski | Lubusz Voivodeship | 66-400 | Poland |
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| ID | Term |
|---|---|
| D019964 | Mood Disorders |
| D044342 | Malnutrition |
| ID | Term |
|---|---|
| D001523 | Mental Disorders |
| D009748 | Nutrition Disorders |
| D009750 | Nutritional and Metabolic Diseases |
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| POMS | Profile of Mood States | day before the test |
| REST-Q sport | Recovery-Stress questionnaire for Athletes | day before the test |
| Time Frame: whole day (24hours) before test, in the morning before the test |
| fat | fat [g ]of food intake measured by food diary | Time Frame: whole day (24hours) before test, in the morning before the test |
| carbohydrate | carbohydrate [g ]of food intake measured by food diary | Time Frame: whole day (24hours) before test, in the morning before the test |
| fiber | fiber [g ]of food intake measured by food diary | Time Frame: whole day (24hours) before test, in the morning before the test |
| Body mass | Body mass [kg] measured by electronic scale to the nearest 0,05 kg (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan) | in the morning before the test up to 1 hour after getting up |
| Body fat | Body fat [%] measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan) | in the morning before the test up to 1 hour after getting up |
| Total body water | Body water [%] measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan) | in the morning before the test up to 1 hour after getting up |
| Lean body mass | Lean body mass [kg] measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan) | in the morning before the test up to 1 hour after getting up |
| Height | [cm] measured by high meter | in the morning before the test up to 1 hour after getting up |
| Self reported scale of gastrointestinal symptoms e.g.burping, heartburn, flatulence, abdominal pain, the urge to reiterate, vomiting, diarrhoea, nausea, dizziness, and stitch | 10-point scale where 10 was the symptom with the greatest severity | up to 1 hours after the test |