The Effect of Coffee on Energy Expenditure and Caffeine Metabolism

The Effect of Coffee on Energy Expenditure and Caffeine Metabolism

Obesity is a risk factor for chronic diseases, and improving energy expenditure is a way to prevent and treat obesity. The purpose of this project is to explore the relationship between the effect of coffee on energy expenditure and caffeine metabolism, which has important significance in the rational application of caffeine.

The investigators will study the relationship between the effect of coffee on energy expenditure and caffeine metabolism. The investigators will recruit healthy participants. Participants will be forbidden to consume stimulant drinks such as coffee for 24 hours before the experiment. When the participants arrive at the lab on the day of the experiment, they will sign an informed consent form, a health history questionnaire and a daily coffee consumption questionnaire. The changes of drinking water/coffee on total energy expenditure (TEE), body temperature, heart rate, blood pressure, electrocardiogram, memory，cognition, sleep time and so on will be observed. The concentration of caffeine and its metabolites in the blood and urine will be measured by liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS).

Inclusion Criteria：

• Healthy adults.
• Must be able to drink coffee.

Exclusion Criteria:

• Diabetes.
• Hypoglycemia.
• Gout.
• Osteoporosis.
• Gastric ulcer.
• Pancreatitis.
• Intestinal obstruction.
• Blood phobia.
• Pathological hypo or hyper tension.
• Impaired glucose tolerance.
• Claustrophobia.
• HIV,etc.