Group (Ⅰ) (non-diabetic patients): n=10 subdivided into Test side: received scaling and rootplanning plus the subgingival application of PRP.

Control side: received scaling and rootplanning only.

Group (Ⅱ) (non-diabetic patients): n=10 subdivided into Test side: received MD plus subgingival application of I-PRF. Control side: received MD only.

Group (Ⅲ) (diabetic patients): n=10 subdivided into Test side: received MD plus subgingival application of PRP. Control side: received MD only.

Group(Ⅳ) (diabetic patients): n=10 subdivided into Test side: received MD plus the subgingival application of I-PRF Control side: received MD only.

At the 1st visit, each quadrant is scaled and root planned using an ultrasonic scaler and manual instruments until tactile sensation detects smooth crown and root surfaces these procedures were repeated on both sides starting from 1st visit of treatment,2nd,4th, and 6th-week intervals local anesthetic was utilized in some instances where it was the necessary and subgingival application of (PRP) at test side only at baseline,3ed, 6th week.

It assesses oral hygiene and is based on the recording of soft debris on the teeth, plaque was scored using the plaque indices the Tarski et al Modified Quigley Hein Plaque Index (TQHPI). With the TQHPI, mesial, distal, and mid surfaces of facial and lingual aspects were scored:

0 = no plaque/debris

1. = separate flecks of plaque at the cervical margin of the tooth.
2. = a thin continuous band of plaque (up to 1 mm) at the cervical margin of the tooth.
3. = a band of plaque wider than 1 mm but covering less than one-third of the crown of the tooth.
4. = plaque covering at least one-third but less than two-thirds of the crown of the tooth.
5. = plaque covering two-thirds or more of the crown of the tooth. The index for the tooth was calculated by adding the scores from the six surfaces of the tooth and dividing them by six. For each patient, a plaque index was calculated by adding up the indices for each of the teeth examined and dividing by the number of these teeth.

In 1971, Muhlemann & Son introduced the SBI, which was defined as follows:

Score 0 gingiva of normal texture and color, no bleeding. Score 1: gingiva normal, bleeding on probing. Score 2: bleeding on probing, change in color, no edema. Score 3: bleeding on probing change in color, slight oedema. Score 4: either: (a) bleeding on probing, change in color, obvious edema or (b) bleeding on probing, obvious edema.

Score 5: bleeding on probing and spontaneous bleeding, change in color, marked edema.

For all groups, the pocket depth measurement was calculated from the gingival margin to the base of the pocket in each quadrant between the first premolar and 1ST molar. It was taken at 6 locations which were mesiofacial, midfacial, distofacial, mesiolingual, midlingual, and distolingual.

To the closest millimeter, the standard William's graded probe was employed. It was inserted parallel to the tooth's long axis with light force; if present, any heavy calculus deposits interfering with measurement were removed.

The measurements were put together before being divided by six to determine the mean value of every tooth.

Following that, the pocket depth value for each patient was calculated by summing the values of all teeth, and then the total number of teeth tested divided by the sum.

Both periodontal pathogens Porphyromonas gingivalis (Pg) and Prevotella intermedia (PI) were detected within subgingival dental plaque samples at baseline before treatment, 6th, and 12th weeks by semiquantitative culture technique, and the resulting bacteria were identified using (PCR).• Quantification of each organism was done by counting the number of colonies forming units (CFU) on each plate. Because dilutions are based on 1 paper point, the plate count times the dilution factor yielded the total number of organisms per paper point A significant reduction was considered when the CFU/paper point decreased to ≤103; a non-significant reduction was considered when the CFU/paper point was 103-105; and a failure of treatment was considered when the CFU/paper point remained ≥105.