Injectable PRF in Non-Surgical Periodontal Treatment

Adjunctive Use of Injectable PRF in Non-surgical Periodontal Therapy: Clinical and Microbiological Outcomes ( Split - Mouth Randomized Controlled Trial )

This study aims to evaluate the clinical and microbiological effects of using injectable Platelet-Rich Fibrin (i-PRF) as an adjunct to non-surgical periodontal therapy (scaling and root planing) in patients with periodontitis. The study uses a split-mouth design where one side of the mouth receives the treatment with i-PRF and the other side receives standard treatment alone. Clinical parameters and bacterial counts will be compared at baseline, 1 month, and 3 months after intervention .

Patients with periodontitis will be recruited for this split-mouth randomized controlled trial. All participants will receive full-mouth supra and sub-gingival scaling and root planing (SRP). In the intervention side, i-PRF will be injected into the periodontal pockets, while the control side will receive SRP only.

Clinical measurements including Probing Pocket Depth (PPD), Clinical Attachment Level (CAL), and Bleeding on Probing (BOP) will be recorded. Additionally, microbiological samples will be collected to assess the changes in the subgingival Porphyromonas gingivalis and Fusobacterium nucleatum . Follow-up visits are scheduled at 1 and 3 months post-intervention to assess the adjunctive benefit of i-PRF.

Inclusion Criteria:

• Adult subjects aged 30 years and above.
• No history of systemic diseases (e.g., diabetes mellitus).
• Not currently under active periodontal therapy or joining other trial in the last 3 months.
• Diagnosis with Stage II or III Periodontitis.
• presence of interdental CAL affecting ≥2 non adjacent teeth or if CAL at buccal/lingual aspects associated with PPD >4mm at ≥2 teeth.

Exclusion Criteria:

• Smokers or alcohol users.
• Patients consuming antibiotics or anticoagulants.
• Regular users of non-steroidal anti-inflammatory drugs.
• Receipt of periodontal treatment within 3 months prior to the study.
• Any bleeding or clotting disorders.
• Teeth with Grade II or Grade III furcation involvement.
• Pregnant or breastfeeding mothers.
• Patients unwilling to participate.