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Rationale: Collagen protein is the central structural component of extracellular connective tissues within skeletal muscle, bone, cartilage and skin. Dietary collagen peptides are a promising protein source to deliver the specific amino acid precursors required to support an increase in connective tissue protein synthesis across several tissues (e.g. muscle, skin). However, the digestion and absorption kinetics of multiple boluses of collagen peptides and the subsequent impact on muscle and skin connective tissue protein synthesis rates have not yet been assessed in vivo in humans.
Objective: To assess the impact of ingestion of multiple boluses of collagen peptides on muscle connective and skin protein synthesis in vivo in humans.
Study design: Double-blind, parallel-group, placebo-controlled intervention study.
Study population: 20 healthy young males, aged 18-35 years.
Intervention : Participants will perform unilateral resistance exercise followed by the ingestion of either 100 g of collagen peptides (in boluses) or a non-caloric placebo (flavoured water) drinks, while all drinks will contain vitamin C. Continuous intravenous stable isotope amino acid tracer infusions will be applied, plasma, skin and muscle samples will be collected in order to assess protein synthesis rates in skin and muscle tissue.
Main study parameters/endpoints: Primary study parameters are muscle connective protein synthesis rates. Secondary study parameters are skin and myofibrillar protein synthesis rates, plasma amino acid concentrations and body composition.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Collagen protein | Experimental | 100 g of collagen peptides (in boluses) with vitamin C |
|
| Placebo | Placebo Comparator | Non-caloric placebo (flavoured water) drinks, with vitamin C. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Collagen protein | Dietary Supplement | Ingestion of 40, 20, 20 and 20 gram of collagen protein right after and every 2 hours after a bout of exercise |
|
| Measure | Description | Time Frame |
|---|---|---|
| Muscle connective protein synthesis rates rested leg | The primary analysis will be an independent t-test, comparing postprandial muscle connective protein synthesis rates over the 0-8 h period (i.e., one integrated value) in the rested condition between groups. | one value calculated over 8 hours |
| Measure | Description | Time Frame |
|---|---|---|
| Muscle connective protein synthesis rates exercised leg | Independent t-test, comparing postprandial muscle connective protein synthesis rates over the 0-8 h period (i.e., one integrated value) in the exercised condition between groups. | one value calculated over 8 hours |
| Myofibrillar protein synthesis rates rested leg |
| Measure | Description | Time Frame |
|---|---|---|
| Age | age in years | baseline |
| Dietary macronutrient intake | assessed by written dietary intake records | 2 days before test days |
Inclusion Criteria:
Exclusion Criteria:
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Luc JC van Loon, PhD | Contact | +31-43-3881397 | l.vanloon@maastrichtuniversity.nl |
| Name | Affiliation | Role |
|---|---|---|
| Luc JC van Loon, PhD | Maastricht University | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Maastricht University Medical Centre | Maastricht | 6200 MD | Netherlands |
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| ID | Term |
|---|---|
| D055070 | Resistance Training |
| ID | Term |
|---|---|
| D005081 | Exercise Therapy |
| D012046 | Rehabilitation |
| D000359 | Aftercare |
| D003266 | Continuity of Patient Care |
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Double-blind, parallel-group, placebo-controlled intervention study
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| Resistance exercise | Behavioral | A single resistance exercise session of the leg press and leg extension exercise |
|
Independent t-test, comparing postprandial myofibrillar protein synthesis rates over the 0-8 h period (i.e., one integrated value) in the rested condition between groups. |
| one value calculated over 8 hours |
| Myofibrillar protein synthesis rates exercised leg | Independent t-test, comparing postprandial myofibrillar protein synthesis rates over the 0-8 h period (i.e., one integrated value) in the exercised condition between groups. | one value calculated over 8 hours |
| Skin protein synthesis rates | Independent t-test, comparing postprandial skin protein synthesis rates over the 0-8 h period (i.e., one integrated value). | one value calculated over 8 hours |
| Plasma insulin concentrations | Plasma insulin concentrations | measured over the 8 hour post-prandial period |
| Plasma amino acids concentrations | Plasma amino acids concentrations | measured over the 8 hour post-prandial period |
| Body mass | body mass in kg | baseline |
| Height | Height in m | baseline |
| Lean mass | Measured by DXA | baseline |
| One repetition maximum of the legs | maximum strength of both legs | baseline |
| D005791 |
| Patient Care |
| D013812 | Therapeutics |
| D026741 | Physical Therapy Modalities |
| D064797 | Physical Conditioning, Human |
| D015444 | Exercise |
| D009043 | Motor Activity |
| D009068 | Movement |
| D009142 | Musculoskeletal Physiological Phenomena |
| D055687 | Musculoskeletal and Neural Physiological Phenomena |