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The purpose of this study is to assess which amino acids are affecting acute muscle collagen synthesis in response to supplementation and exercise.
This is a double-blinded, randomized controlled, parallel design study, with 3 treatment groups (placebo, collagen peptide, and essential amino acid). The study will involve 3 visits: a baseline testing/familiarization session, and 2 exercise trials which will include muscle biopsies, blood draws, and the infusion of a tracer isotope in order to measure muscle protein synthesis.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Essential Amino Acid | Active Comparator | Participants will consume 1 dose of: 15 g crystalline essential amino acid supplement (Pure Encapsulation Essential Aminos 180) + 80 ml (48 mg) Vitamin C (Ribena Blackcurrant) + 250 ml water |
|
| Collagen Peptide | Active Comparator | Participants will consume 1 dose of: 15g collagen peptide (Gelita TENDOFORTE) + 80 ml (48 mg) Vitamin C (Ribena Blackcurrant) + 250 ml water |
|
| Maltodextrin Placebo | Placebo Comparator | Participants will consume 1 dose of:15 g maltodextrin (Canadian Protein Maltodextrin) + 80 ml (48 mg) Vitamin C (Ribena Blackcurrant) + 250 ml water |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Essential Amino Acid | Dietary Supplement | Immediately following the exercise intervention participants will consume their assigned essential amino acid supplement |
|
| Measure | Description | Time Frame |
|---|---|---|
| Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis | Fraction of the myofibrillar enriched protein from muscle biopsy per unit time. | 0 - 4 hours post-exercise |
| Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis | Fraction of the myofibrillar enriched protein from muscle biopsy per unit time. | 0 - 2 hours post-exercise |
| Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis | Fraction of the myofibrillar enriched protein from muscle biopsy per unit time. | 2 - 4 hours post-exercise |
| Fractional synthetic rate (change over time) Muscle Collagen Synthesis | Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time. | 0 - 4 hours post-exercise |
| Fractional synthetic rate (change over time) Muscle Collagen Synthesis | Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time. | 0 - 2 hours post-exercise |
| Fractional synthetic rate (change over time) Muscle Collagen Synthesis | Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time. | 2 - 4 hours post-exercise |
| Pro-Collagen Type I N-Propeptide (PINP) | Osteoblastic marker of bone collagen synthesis |
| Measure | Description | Time Frame |
|---|---|---|
| Plasma Amino Acids Response to Supplementation | Amino acid plasma concentrations | 0 - 4 hours post-exercise |
| Muscle mechanistic target of rapamycin Pathway Activation | Measuring the fold change in enzymes associated with the anabolic signalling of the mTOR pathway (e.g. phosphoinositide 3-kinases (PI3k), protein kinase B (Akt)) |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Cameron Mitchell, PhD | University of British Columbia | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Chan Gunn Pavilion | Vancouver | British Columbia | V6T 1Z3 | Canada |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 27852613 | Background | Shaw G, Lee-Barthel A, Ross ML, Wang B, Baar K. Vitamin C-enriched gelatin supplementation before intermittent activity augments collagen synthesis. Am J Clin Nutr. 2017 Jan;105(1):136-143. doi: 10.3945/ajcn.116.138594. Epub 2016 Nov 16. | |
| 27603578 | Background | Holm L, Rahbek SK, Farup J, Vendelbo MH, Vissing K. Contraction mode and whey protein intake affect the synthesis rate of intramuscular connective tissue. Muscle Nerve. 2017 Jan;55(1):128-130. doi: 10.1002/mus.25398. Epub 2016 Sep 26. |
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- Double-blinded, randomized controlled, parallel design study, with 3 treatment groups (placebo, collagen peptide, and essential amino acid)
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| Collagen Peptide | Dietary Supplement | Immediately following the exercise intervention participants will consume their assigned collagen peptide supplement |
|
| Maltodextrin Placebo | Dietary Supplement | Immediately following the exercise intervention participants will consume their assigned maltodextrin supplement |
|
| 1 hour prior to exercise |
| Pro-Collagen Type I N-Propeptide (PINP) | Osteoblastic marker of bone collagen synthesis | 4 hours post exercise |
| 0 - 4 hours post-exercise |
| PCR Gene Expression of Muscle Collagen Genes (Types I, III, IV) | Measuring the fold change when the collagen genes of interest become detectable in the PCR analysis | 0 - 4 hours post-exercise |
| Pro-Collagen Type III N-Propeptide (PIIINP) | Marker of collagen synthesis | 1 hour prior to exercise |
| Pro-Collagen Type III N-Propeptide (PIIINP) | Marker of collagen synthesis | 4 hours post exercise |
| 25294644 | Background | Frontera WR, Ochala J. Skeletal muscle: a brief review of structure and function. Calcif Tissue Int. 2015 Mar;96(3):183-95. doi: 10.1007/s00223-014-9915-y. Epub 2014 Oct 8. |
| 24237131 | Background | Egerman MA, Glass DJ. Signaling pathways controlling skeletal muscle mass. Crit Rev Biochem Mol Biol. 2014 Jan-Feb;49(1):59-68. doi: 10.3109/10409238.2013.857291. Epub 2013 Nov 18. |
| 19903866 | Background | Holm L, van Hall G, Rose AJ, Miller BF, Doessing S, Richter EA, Kjaer M. Contraction intensity and feeding affect collagen and myofibrillar protein synthesis rates differently in human skeletal muscle. Am J Physiol Endocrinol Metab. 2010 Feb;298(2):E257-69. doi: 10.1152/ajpendo.00609.2009. Epub 2009 Nov 10. |
| 31681789 | Background | Alcock RD, Shaw GC, Tee N, Burke LM. Plasma Amino Acid Concentrations After the Ingestion of Dairy and Collagen Proteins, in Healthy Active Males. Front Nutr. 2019 Oct 15;6:163. doi: 10.3389/fnut.2019.00163. eCollection 2019. |
| 16002437 | Background | Miller BF, Olesen JL, Hansen M, Dossing S, Crameri RM, Welling RJ, Langberg H, Flyvbjerg A, Kjaer M, Babraj JA, Smith K, Rennie MJ. Coordinated collagen and muscle protein synthesis in human patella tendon and quadriceps muscle after exercise. J Physiol. 2005 Sep 15;567(Pt 3):1021-33. doi: 10.1113/jphysiol.2005.093690. Epub 2005 Jul 7. |
| ID | Term |
|---|---|
| D009043 | Motor Activity |
| ID | Term |
|---|---|
| D001519 | Behavior |
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| ID | Term |
|---|---|
| D000601 | Amino Acids, Essential |
| ID | Term |
|---|---|
| D000596 | Amino Acids |
| D000602 | Amino Acids, Peptides, and Proteins |
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