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The study aims to evaluate the metabolizable energy of the typical American diet with and without the inclusion of fiber. We hypothesize that including fiber in the diet will decrease the metabolizable energy of the diet resulting in more nutrient loss into the feces and urine.
The study will be a randomized, crossover, controlled feeding trial comprised of 2 experimental periods: 1) control and 2) fiber. Each experimental condition will be 16 days in length. There will be at least a 12-day compliance break between each period. Participants will undergo complete urine and fecal collection for a period five to seven days during the second half of each experimental condition.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Intervention Treatment | Experimental | Experimental treatment will contain a cereal product that contains a dietary fiber. This will be consumed three times daily for two weeks. |
|
| Control treatment | Placebo Comparator | Control treatment will contain a corn meal cereal product that will be consumed three times daily for two weeks. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Fiber | Other | The intervention treatment will contain fiber. |
| |
| Measure | Description | Time Frame |
|---|---|---|
| Metabolizable Energy of the Diet | The diets provided to participants will be analyzed for their energy and proximate analysis. Fecal and urine samples will be collected for 5 days during week 2 of each intervention period to calculate the total metabolizable energy of the diet. Energy and proximate analysis (dry matter, organic matter, crude protein, acid-hydrolyzed fat, and total dietary fiber) will be analyzed from the provided diet and the fecal samples. Energy will be analyzed from urine samples. The metabolizable energy content will be calculated according to the methods of Novotny et al. (2012), which considers the energy of the diet compared to the remaining energy in the collected fecal and urine samples. | during the last 5 days of the 2 week intervention period |
| Measure | Description | Time Frame |
|---|---|---|
| Fecal Microbiota | We will conduct qPCR to quantify total bacterial cells within one fresh fecal sample collected during week 2 of each condition. | end of week 2 (end of intervention period) |
| Measure | Description | Time Frame |
|---|---|---|
| Gastrointestinal Tolerance | Participants will indicate if tolerance symptoms (burping, cramping/pain, distension/bloating, flatulence/gas, nausea, reflux, rumblings) are absent, mild, moderate, or severe during the second week of each intervention period. All measures will be assessed using a 4-point scale (1=absent, 2=mild, 3=moderate, 4=severe) | daily during the second week of each intervention period |
Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Edward R Madigan Laboratory | Urbana | Illinois | 61801 | United States |
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| ID | Term |
|---|---|
| D005247 | Feeding Behavior |
| ID | Term |
|---|---|
| D001522 | Behavior, Animal |
| D001519 | Behavior |
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| ID | Term |
|---|---|
| D004043 | Dietary Fiber |
| ID | Term |
|---|---|
| D004040 | Dietary Carbohydrates |
| D002241 | Carbohydrates |
| D005502 | Food |
| D000066888 | Diet, Food, and Nutrition |
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| Control |
| Other |
The control treatment will contain corn meal. |
|
| Bowel Function | Participants will indicate number (how many) and time of bowel movements during week 2 of each intervention condition. | week 2 of each intervention period |
| Stool Consistency | Participants will use the Bristol Stool Scale during week 2 of each intervention condition to indicate their stool consistency. | daily during the second week of each intervention period |
| Ease of Passage | Participants will indicate the ease of passage of each bowel movement during week 2 of each intervention condition. The 0-5 scale ranges from 1 (very easy) to 5 (very difficult). | daily during the second week of each intervention period |
| D010829 |
| Physiological Phenomena |
| D019602 | Food and Beverages |