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Participants will ask to answer questionnaire related to general characteristic, lifestyle, dietary pattern and undergo physical examination and measurement of body composition. The subjects will be divided into 2 groups: healthy control and intervention. The intervention group was divided into 3 subgroups: the fermented vegetable (kimchi) group, the kimchi supplemented with polyphenol (Vitexin) group, or the kimchi supplemented with probiotic (Lactobacillus rhamnosus) group. The intervention group will be instructed to consume 1 pack of kimchi per day (50 g/pack) with lunch for 2 months.
The subjects will be divided into 2 groups: healthy control and intervention. The intervention group was divided into 3 subgroups: the fermented vegetable (kimchi) group, the kimchi supplemented with polyphenol (Vitexin) group, or the kimchi supplemented with probiotic (Lactobacillus rhamnosus) group. The intervention group will be instructed to consume 1 pack of kimchi per day (50 g/pack) with lunch for 2 months. During the study, participants will be strongly encouraged to avoid consuming prebiotics or probiotic supplements, as well as foods such as yogurt, sour milk, fermented vegetables, and fermented fruits, especially 2 weeks before the blood and fecal examination. Compliance will be assessed by monitoring product side effects through follow-up phone calls every week to check on participants' symptoms and assessing the number of sachets or packs they actually consume.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Supplementation-standard | Active Comparator | Supplementation with Kimchi 50 gram daily for two months |
|
| Supplementation with probiotic | Experimental | Supplementation with Kimchi plus LGG 50 gram daily for two months |
|
| Supplementation with polyphenol | Experimental | Supplementation with Kimchi plus polyphenol 50 gram daily for two months |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Fermented Vegetables (Kimchi) supplementation | Dietary Supplement | Fermented Vegetables (Kimchi) supplementation with 50 gm daily for two months and effects of DNA methylation, serum proteome and microbiota |
| Measure | Description | Time Frame |
|---|---|---|
| ฺBMI | changes in BMI for evaluating obesity at 2 months from baseline | 2 months |
| gut bacterial flora | Changes and differences of the gut bacterial flora will be addressed in detail, e.g. changes in concentration of specific bacterial groups (e.g. Lactobacillus) before and after the intervention period. | 2 months |
| Measure | Description | Time Frame |
|---|---|---|
| DNA methylation of inflammation and anti-inflammation gene | changes in methylation (hypomethylation and hypermethylation) | 2 months |
| Serum proteome | changes in protein expression (up and dow-regulation) |
| Measure | Description | Time Frame |
|---|---|---|
| The differences between the treatment groups and the non-obese group for exploratory variables | Changes in blood biochemistry (lipid profile, inflammatory cytokine level), protein expression (up and dow-regulation), %methylation (hypomethylation and hypermethylation) and concentration of specific bacterial groups | baseline |
Inclusion Criteria: for the intervention group
Exclusion Criteria:for the intervention group
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Jintana Sirivarasai, Ph.D | Contact | 662-201-1483 | jintana.sir@mahidol.ac.th | |
| Prapimporn Chattranukulchai, MD | Contact | 662-201-1922 | sprapimporn@gmail.com |
| Name | Affiliation | Role |
|---|---|---|
| Jintana Sirivarasai, Ph.D | Ramathibodi Hospital | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Jintana Sirivarasai | Bangkok | 10400 | Thailand |
all IPD that underlie results in a publication
starting 12 months after publication
After summary data are published and others can access via journal website
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| ID | Term |
|---|---|
| D009765 | Obesity |
| ID | Term |
|---|---|
| D050177 | Overweight |
| D044343 | Overnutrition |
| D009748 | Nutrition Disorders |
| D009750 | Nutritional and Metabolic Diseases |
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| ID | Term |
|---|---|
| D019587 | Dietary Supplements |
| D019936 | Probiotics |
| D059808 | Polyphenols |
| ID | Term |
|---|---|
| D005502 | Food |
| D000066888 | Diet, Food, and Nutrition |
| D010829 | Physiological Phenomena |
| D019602 | Food and Beverages |
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|
| probiotic | Dietary Supplement | Supplementation with Kimchi plus LGG 50 grams daily for two months |
|
| polyphenol | Dietary Supplement | Supplementation with Kimchi plus polyphenol 50 grams daily for two months |
|
| 2 months |
| D001835 |
| Body Weight |
| D012816 | Signs and Symptoms |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D010636 |
| Phenols |
| D001555 | Benzene Derivatives |
| D006841 | Hydrocarbons, Aromatic |
| D006844 | Hydrocarbons, Cyclic |
| D006838 | Hydrocarbons |
| D009930 | Organic Chemicals |