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The present study aims to evaluate in adults with normal weight and with overweight or obesity who live in the city of La Plata the circulating levels of various hormones that regulate appetite and the gene expression of their receptors in peripheral blood mononuclear cells before and after food intake.
the investigators plan a single test meal study in males and females with normal weight (NW) or overweight/obesity (OW/OB) assessing whether plasma appetite regulatory hormones levels are associated with different metabolic and anthropometric variables as well as with appetite sensations.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| food intake (breakfast) | Experimental | the breakfast composition is 56 kcal% carbohydrates, 12 kcal% protein and 34 kcal% lipids and comprises yogurt, cereals and almonds |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| food (breakfast) | Dietary Supplement | The breakfast composition is 56 kcal% carbohydrates, 12 kcal% protein and 34 kcal% lipids and comprises yogurt, cereals and almonds |
|
| Measure | Description | Time Frame |
|---|---|---|
| plasma ghrelin concentration | ghrelin concentration (fmol/ml) | pre -prandial |
| plasma leptin concentration | leptin concentration (ug/ml) | pre -prandial |
| plasma glucagon-like peptide 1 concentration | glucagon-like peptide 1 concentration (ug/ml) | pre -prandial |
| plasma liver antimicrobial peptide 2 concentration | liver antimicrobial peptide 2 concentration (pmol/ml) | pre -prandial |
| plasma des-acyl ghrelin concentration | des-acyl ghrelin concentration (fmol/ml) | pre -prandial |
| plasma ghrelin concentration | ghrelin concentration (fmol/ml) | 1 hour post -prandial |
| plasma leptin concentration | leptin concentration (ug/ml) | 1 hour post -prandial |
| plasma glucagon-like peptide 1 concentration | glucagon-like peptide 1 concentration (ug/ml) | 1 hour post -prandial |
| plasma liver antimicrobial peptide 2 concentration |
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Inclusion Criteria:
Exclusion Criteria:
Pregnancy
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| Name | Affiliation | Role |
|---|---|---|
| Maria F Andreoli | Instituto de Desarrollo e Investigaciones Pediátricas (IDIP) | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Instituto de Desarrollo e Investigaciones Pediátricas (IDIP) | La Plata | Buenos Aires | 1900 | Argentina |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 38157050 | Derived | Andreoli MF, Fittipaldi AS, Castrogiovanni D, De Francesco PN, Valdivia S, Heredia F, Ribet-Travers C, Mendez I, Fasano MV, Schioth HB, Doi SA, Habib AM, Perello M. Pre-prandial plasma liver-expressed antimicrobial peptide 2 (LEAP2) concentration in humans is inversely associated with hunger sensation in a ghrelin independent manner. Eur J Nutr. 2024 Apr;63(3):751-762. doi: 10.1007/s00394-023-03304-8. Epub 2023 Dec 29. |
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| ID | Term |
|---|---|
| D009765 | Obesity |
| D001070 | Appetitive Behavior |
| ID | Term |
|---|---|
| D050177 | Overweight |
| D044343 | Overnutrition |
| D009748 | Nutrition Disorders |
| D009750 | Nutritional and Metabolic Diseases |
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| ID | Term |
|---|---|
| D005502 | Food |
| D062408 | Breakfast |
| ID | Term |
|---|---|
| D000066888 | Diet, Food, and Nutrition |
| D010829 | Physiological Phenomena |
| D019602 | Food and Beverages |
| D062407 | Meals |
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Participants will be given a test meal (breakfast) that provides 20% of the individual's daily energy requirement. The breakfast composition is 56 kcal% carbohydrates, 12 kcal% protein and 34 kcal% lipids and comprises yogurt, cereals and almonds. Participants will be asked to consume the entire breakfast in less than 15 min and to complete a visual analog scale (VAS) questionnaire every 15 min. Blood sampling will be repeated at 60 min after starting breakfast (postprandial).
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liver antimicrobial peptide 2 concentration (pmol/ml) |
| 1 hour post -prandial |
| plasma des-acyl ghrelin concentration | des-acyl ghrelin concentration (fmol/ml) | 1 hour post -prandial |
| plasma glucose concentration | glucose concentration (mg%) | pre -prandial |
| plasma glucose concentration | glucose concentration (mg%) | 1 hour post -prandial |
| plasma triglycerides concentration | triglycerides concentration (mg%) | pre-prandial |
| plasma triglycerides concentration | triglycerides concentration (mg%) | 1 hour post -prandial |
| plasma total cholesterol concentration | total cholesterol concentration (mg%) | pre -prandial |
| plasma total cholesterol concentration | total cholesterol concentration (mg%) | 1 hour post -prandial |
| ghrelin proteolysis | plasma proteolytic activity of ghrelin (relative units) | pre -prandial |
| ghrelin proteolysis | plasma proteolytic activity of ghrelin (relative units) | 1 hour post -prandial |
| ghrelin deacylation | plasma desacylase actvitity of ghrelin (relative units) | pre -prandial |
| ghrelin deacylation | plasma desacylase actvitity of ghrelin (relative units) | 1 hour post -prandial |
| leptin receptor gene expression in peripheral blood mononuclear cells | leptin receptor gene expression in peripheral blood mononuclear cells (relative expression) | pre-prandial |
| insulin receptor gene expression in peripheral blood mononuclear cells | insulin receptor gene expression in peripheral blood mononuclear cells (relative expression) | pre-prandial |
| ghrelin receptor gene expression in peripheral blood mononuclear cells | ghrelin receptor gene expression in peripheral blood mononuclear cells (relative expression) | pre-prandial |
| hunger sensation | visual analog scale (cm) | pre-prandial and 15, 30, 45 and 60 min post-prandial |
| fullness sensation | visual analog scale (cm) | pre-prandial and 15, 30, 45 and 60 min post-prandial |
| "desire to eat" sensation | visual analog scale (cm) | pre-prandial and 15, 30, 45 and 60 min post-prandial |
| "prospective food consumption" sensation | visual analog scale (cm) | pre-prandial and 15, 30, 45 and 60 min post-prandial |
| D001835 |
| Body Weight |
| D012816 | Signs and Symptoms |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D001522 | Behavior, Animal |
| D001519 | Behavior |