Establishment of a Method for Extraction and Quantification of Gingival Tissue Nicotine y

Establishment of a Method for Extraction and Quantification of Gingival Tissue Nicotine in Relation to Conventional Tobacco Biomarkers: a Pilot Study

establishment of a method for extraction and quantification of gingival tissue nicotine in relation to conventional tobacco biomarkers: a pilot study

The study aim to establish a method for extraction and quantification of gingival tissue nicotine. Additionnaly, the relationship between the nicotine tissue intoxication with others smoking indicators will be evaluated as mentionned in the following PICO question:

• P: Smoker patients
• I: Biopsie of gingival tissue
• C: Non-smoker patient
• O: nicotine dependence test (FTND), salivary cotinine, fibroblast nicotine, number of cigarette consumed per day (NCC) and the pack-year (PY)

Inclusion Criteria:

• non smokers
• smokers

Exclusion Criteria:

• not able to have biopsie