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| Name | Class |
|---|---|
| National Natural Science Foundation of China | OTHER_GOV |
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To study the molecular pathogenesis of cataract and explore possible treatments，anterior lens capsules were collected from cataract patients
Anterior lens capsules were collected from the patients with DC and ARC, and transparent crystals of cadaveric eyes. M6A epitranscriptomic microarray was performed to investigate the altered m6A modifications and determine the differentially expressed mRNAs. The potential role of dysregulated m6A modification levels was predicted through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. Real-time polymerase chain reaction was performed to identify the dysregulated expression of RNA methyltransferases, demethylases, and readers.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| DC group | Anterior lens capsule tissues from DC patients | ||
| ARC group | Anterior lens capsule tissues from ARC patients | ||
| NC group | anterior lens capsules collected from three age-matched transparent crystals of cadaveric eyes |
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| Measure | Description | Time Frame |
|---|---|---|
| m6A modification of mRNAs | Epitranscriptomic microarray analysis reveals the differential m6A modification of mRNAs in DC samples | 1 month |
| expression level of mRNAs | the m6A microarray analyses provide data for mRNA expression levels | 1 month |
| levels of methylated mRNA | in vitro validation of the diverse expression levels of methylated mRNA | 1 month |
| levels of RNA methyltransferase | in vitro validation of the diverse expression levels of RNA methyltransferase | 1 month |
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Inclusion Criteria:
Exclusion Criteria:
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Anterior lens capsule tissues from cataract patients with diabetes mellitus more than 10 years, patients with age related cataract, and transparent crystals of cadaveric eyes.
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Lei Cai, MD | Contact | 13816956828 | clyc0318@126.com |
| Name | Affiliation | Role |
|---|---|---|
| Jin Yang, PhD | Eye & ENT Hospital | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Eye & ENT Hospital of Fudan University | Recruiting | Shanghai | Shanghai Municipality | 200031 | China |
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| ID | Term |
|---|---|
| D002386 | Cataract |
| ID | Term |
|---|---|
| D007905 | Lens Diseases |
| D005128 | Eye Diseases |
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Anterior lens capsule tissues from cataract patients