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| ID | Type | Description | Link |
|---|---|---|---|
| FBW/06/2025 | Other Grant/Funding Number | Military Institute of Medicine - National Research Institute | |
| FBW/06/2025 | Other Grant/Funding Number | University of Warsaw |
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This observational study will compare proteomic and elemental profiles of aqueous humor and ocular tissues in patients with primary open-angle glaucoma (POAG) and cataract versus cataract controls without glaucoma. Samples collected during standard surgery will be analyzed using LC-MS/MS and ICP-MS. The goal is to identify glaucoma-associated molecular patterns with potential diagnostic or prognostic relevance.
This observational study will evaluate molecular and elemental differences in aqueous humor and ocular tissues in patients with primary open-angle glaucoma (POAG) and cataract compared with cataract controls without glaucoma. Samples of aqueous humor (~100 µL) will be collected intraoperatively from the anterior chamber, and ocular tissues routinely removed during surgery (anterior lens capsule and, in the glaucoma group, scleral tissue) will be obtained for laboratory analyses.
Participants will undergo standard clinical assessment including intraocular pressure (IOP), optic nerve head evaluation, optical coherence tomography (OCT) with retinal nerve fiber layer (RNFL) analysis, and automated perimetry (Humphrey SITA Standard 24-2). Elemental profiling will be performed using ICP-MS (with LA-ICP-MS mapping in selected samples), and proteomic profiling will be conducted using LC-MS/MS with bioinformatic analysis.
The aim is to identify glaucoma-associated chemical and proteomic patterns that may serve as potential diagnostic or prognostic biomarkers and improve understanding of glaucoma pathophysiology.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Glaucoma + Cataract Group | Experimental |
| |
| Cataract Control Group | Active Comparator |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Phacoemulsification With Intraocular Lens Implantation and Deep Sclerectomy | Procedure | Standard-of-care combined cataract surgery (phacoemulsification with intraocular lens implantation) performed together with deep sclerectomy for glaucoma management. Intraoperative collection of aqueous humor and routinely removed ocular tissues (such as sclera and anterior lens capsule) is performed for research analyses. |
| Measure | Description | Time Frame |
|---|---|---|
| IOP | Intraocular Pressure | Through study completion, an average of 1 year |
| Visual Field | Visual Field of the mean defect (MD) | Through study completion, an average of 1 year |
| BCVA | Best-corrected visual acuity | Through study completion, an average of 1 year |
| Protein expression | Measured by ICP-MS/LC-MS | Through study completion, an average of 1 year |
| RNFL | Retinal Nerve Fiber Layer (OCT) | Through study completion, an average of 1 year |
| Measure | Description | Time Frame |
|---|---|---|
| Medications | Number of medications taken during the study | Through study completion, an average of 1 year |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Marek Rękas, MD, PhD | Contact | 0048665 707 626 | mrekas@wim.mil.pl | |
| Wojciech Mazurek, MD | Contact | 0048261 816 575 | wmazurek@wim.mil.pl |
| Name | Affiliation | Role |
|---|---|---|
| Marek Rękas, MD, PhD | Military Institute od Medicine National Research Institute | Principal Investigator |
| Ewa Bulska, PhD | University of Warsaw | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Military Institute of Medicine - National Research Institute | Recruiting | Warsaw | Poland |
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Parallel-group design comparing patients with glaucoma and cataract to cataract-only controls. Biological samples are collected intraoperatively during standard-of-care surgery for laboratory analysis.
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| Phacoemulsification With Intraocular Lens Implantation | Procedure | Standard-of-care cataract surgery (phacoemulsification with intraocular lens implantation). Intraoperative collection of aqueous humor and routinely removed ocular tissue (anterior lens capsule) is performed for research analyses. |
|
| University of Warsaw | Recruiting | Warsaw | Poland |
|
| ID | Term |
|---|---|
| D005902 | Glaucoma, Open-Angle |
| D002386 | Cataract |
| D005901 | Glaucoma |
| ID | Term |
|---|---|
| D009798 | Ocular Hypertension |
| D005128 | Eye Diseases |
| D007905 | Lens Diseases |
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| ID | Term |
|---|---|
| D018918 | Phacoemulsification |
| D019654 | Lens Implantation, Intraocular |
| ID | Term |
|---|---|
| D002387 | Cataract Extraction |
| D054140 | Refractive Surgical Procedures |
| D013508 | Ophthalmologic Surgical Procedures |
| D013514 | Surgical Procedures, Operative |
| D059708 | Ultrasonic Surgical Procedures |
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