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| Name | Class |
|---|---|
| Selcuk University | OTHER |
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The purpose of this study was to investigate and compare GCF interleukin-1β (IL-1β), interleukin-10 (IL-10), interleukin-36γ (IL-36γ) levels in patients with healthy (H), gingivitis (G) and chronic periodontitis (CP) status.
Gingival crevicular fluid (GCF) is formed when fluid exudes from the vessels of the microcirculation into the inflamed periodontal tissue and into the sulcus or pocket. As the fluid traverses the inflamed tissue, it is thought to pick up enzymes and other molecules that participate in the destructive process, as well as products of cell and tissue degradation. Efforts to develop diagnostic tests based on host factors have been focused almost entirely on analysis of GCF.
The purpose of this study was to investigate and compare GCF interleukin-1β (IL-1β), interleukin-10 (IL-10), interleukin-36γ (IL-36γ) levels in patients with healthy (H), gingivitis (G) and chronic periodontitis (CP) status.
Systemically healthy and non-smokers 80 participants (30 males and 50 females) who were selected from among admitting to Dentistry Faculty of Selcuk University were included in this study. Clinical periodontal parameters were recorded and GCF samples were obtained. Samples were stored in -80 ˚C until analysis with Enzyme-Linked ImmunoSorbent Assay (ELISA).
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Healthy | bleeding on probing (BoP) ≤ 10% of the sites, probing depth (PD) ≤ 3 mm and no indication of clinical attachment level (CAL) or radiographic bone loss |
| |
| Gingivitis | gingival inflammation (BoP ≥ 10% of the sites, red-blue discoloration of the gingiva and edema) PD ≤ 4 mm and no clinical attachment or radiographic bone loss |
| |
| Periodontitis | PD ≤ 5 mm, radiographic bone loss, and 3-4 mm CAL in at least 30% of the sites |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Gingival crevicular fluid sampling | Diagnostic Test | GCF sampling and clinical periodontal measurements were performed |
|
| Measure | Description | Time Frame |
|---|---|---|
| Interleukin-36γ (IL-36γ) levels in GCF | GCF samples were obtained from one interproximal site in each quadrant | Baseline |
| Measure | Description | Time Frame |
|---|---|---|
| Probing depth (PD) | PD; from six sites (mesio-buccal, mid-buccal, disto-buccal, mesio-lingual, mid-lingual, disto-lingual locations) of each tooth other than third molars. | Baseline |
| Clinical attachment level (CAL) |
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Inclusion Criteria:
Exclusion Criteria:
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Patients were selected from among admitting to Dentistry Faculty of Selcuk University.
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| Name | Affiliation | Role |
|---|---|---|
| Mehtikar Gürsel | Bezmialem University | Principal Investigator |
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Results will be shared if requested
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| ID | Term |
|---|---|
| D010510 | Periodontal Diseases |
| ID | Term |
|---|---|
| D009059 | Mouth Diseases |
| D009057 | Stomatognathic Diseases |
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Gingival crevicular fluid
CAL; from six sites (mesio-buccal, mid-buccal, disto-buccal, mesio-lingual, mid-lingual, disto-lingual locations) of each tooth other than third molars.
| Baseline |
| Bleeding on Probing (BoP) | BoP, from six sites (mesio-buccal, mid-buccal, disto-buccal, mesio-lingual, mid-lingual, disto-lingual locations) of each tooth other than third molars | Baseline |
| IL-10 and IL-1β levels in GCF | GCF samples were obtained from one interproximal site in each quadrant | Baseline |
| Plaque Index (PI) | PI; from six sites (mesio-buccal, mid-buccal, disto-buccal, mesio-lingual, mid-lingual, disto-lingual locations) of each tooth other than third molars | Baseline |
| Gingival Index (GI) | GI; from six sites (mesio-buccal, mid-buccal, disto-buccal, mesio-lingual, mid-lingual, disto-lingual locations) of each tooth other than third molars | Baseline |