Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| Name | Class |
|---|---|
| Research Foundation Flanders | OTHER |
Not provided
Not provided
Not provided
Not provided
Blocking histamine H1/H2 receptors blunts chronic endurance training adaptations. The current study addresses a twofold research question: "What is the influence of endurance training (1) and histamine H1 and H2 signaling (2) on the gene expression in human skeletal muscle." Results from this study will yield more insights into the molecular mechanisms of adaptations to exercise training.
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| Label | Type | Description | Intervention Names |
|---|---|---|---|
| 1: Placebo | Placebo Comparator | lactose |
|
| H1 blockade | Experimental | 540 mg Fexofenadine |
|
| H2 blockade | Experimental | 40 mg Famotidine |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Placebo: Placebo + exercise training. | Drug | Oral placebo Exercise training: Interval-based cycling exercise |
|
| Measure | Description | Time Frame |
|---|---|---|
| Change in muscle transcriptome (direct response) | RNA-sequencing | On each test-day: 0 minutes after the exercise training versus at rest. |
| Change in muscle transcriptome (delayed response) | RNA-sequencing | On each test-day: 180 minutes after the exercise training versus at rest. |
| Muscle glycogen depletion | Fluorometric determination of muscle glycogen levels | On each test-day: 0 minutes after the exercise training versus at rest. |
| Muscle glycogen resynthesis | Fluorometric determination of muscle glycogen levels | On each test-day: 180 minutes after the exercise training versus at rest. |
| Plasma volume change | Plasma volume based on hemoglobin and hematocrit concentration. | Change from rest to different time-points after exercise (0, 30, 60, 120 and 180 minutes after the exercise training). |
| Measure | Description | Time Frame |
|---|---|---|
| Heart rate during the exercise training | Heart rate during the exercise training. | Continuously during training on each test-day. |
| Blood lactate | Capillary lactate concentration. |
Not provided
Inclusion Criteria:
Exclusion Criteria:
Not provided
Not provided
Not provided
Not provided
Not provided
| Name | Affiliation | Role |
|---|---|---|
| Wim Derave, Professor | University Ghent | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Department of movement and sports sciences, Ghent University, Belgium | Ghent | Oost-Vlaanderen | 9000 | Belgium |
Not provided
Randomized double-blinded placebo-controlled cross-over interventional study
Not provided
Not provided
Not provided
| H1 blockade: H1 receptor antagonist + exercise training | Drug | H1 blockade: oral blockade Exercise training: Interval-based cycling exercise |
|
| H2 Blockade: H2 receptor antagonist + exercise training | Drug | H2 blockade: oral blockade Exercise training: Interval-based cycling exercise |
|
| On each test-day at 10 time-points: at rest, during exercise and 0, 30, 60, 120 and 180 minutes after the exercise training. |
| Blood glucose | Capillary glucose concentration. | On each test-day at 10 time-points: at rest, during exercise and 0, 30, 60, 120 and 180 minutes after the exercise training. |
| Blood histamine | histamine concentration in blood samples. | On each test-day at 6 time-points: at rest and 0, 30, 60, 120 and 180 minutes after the exercise training. |
| Blood insulin | insulin concentration in blood samples. | On each test-day at 6 time-points: at rest and 0, 30, 60, 120 and 180 minutes after the exercise training. |
| Muscle signaling pathways relevant for glucose metabolism and cardiovascular health | Phosphorylation status of proteins assessed by Western Blotting | On each test-day: at rest, 0 minutes and 180 minutes after the exercise training. |
| ID | Term |
|---|---|
| D009043 | Motor Activity |
| ID | Term |
|---|---|
| D001519 | Behavior |
Not provided
Not provided
| ID | Term |
|---|---|
| D015444 | Exercise |
| ID | Term |
|---|---|
| D009043 | Motor Activity |
| D009068 | Movement |
| D009142 | Musculoskeletal Physiological Phenomena |
| D055687 | Musculoskeletal and Neural Physiological Phenomena |
Not provided
Not provided