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This prospective study the thyroid gland dysfunction and vitamin D receptor polymorphism in keratoconus patients
This is a prospective observational study in which blood samples are collected from keratoconus patients looking for thyroid dysfunction and vitamin D levels in the serum and assessing the Vitamin D receptor polymorphism in comparison to healthy control.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| control group | group of subjects with healthy cornea |
| |
| keratoconus group | group of keratoconus patients with no history of thyroid dysfunction |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| ELISA,DNA extraction | Diagnostic Test | quantitative measurement of (Thyroid stimulating hormon (TSH), free triiodothyronine (FT3)& Free tetraiodothyronine(FT4)) measured using ELISA . Serum 25-OH vitamin D measured using ELISA. Serum 25-OH vitamin D will be classified based on 2012 American Endocrine Society guidelines into deficient (< 20ng/ml), insufficient ( 21 -30 ng/ml) and sufficient (> 30 ng/ml).vitamin D receptor polymorphisms was done in 3 steps: a) DNA extraction from whole blood using Gene Jet gene DNA Purification kits . b) PCR: three Vitamin D receptor polymorphisms were tested [Taq I (restriction enzymes according to single nucleotide polymorphism ) (rs731236), Apa I (rs7975232) and Bsm I (rs1544410)] using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Genomic DNA was amplified using certain primers |
| Measure | Description | Time Frame |
|---|---|---|
| concentration of free T3 | pg/ml | 2month |
| concentration of free T4 | ng/dl | 2 month |
| concentration of TSH | milli-international units per liter | 2 month |
| vitamin D receptor polymorphism | number of participant with vitamin D receptor polymorphism | 2 month |
| Measure | Description | Time Frame |
|---|---|---|
| serum vit D | concentration of Serum 25 OH vitamin D (ng /ml) | 2 month |
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Inclusion Criteria:
Exclusion Criteria:
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outpatient clinic of Mansoura ophthalmic center
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| Name | Affiliation | Role |
|---|---|---|
| Eman Azmy | Mansoura University | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Eman Azmy | Al Mansurah | Dakahlia Governorate | 35511 | Egypt |
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| ID | Term |
|---|---|
| D007640 | Keratoconus |
| ID | Term |
|---|---|
| D003316 | Corneal Diseases |
| D005128 | Eye Diseases |
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5ml venous blood was withdrawn from all subjects included in the study; 2ml on ethylenediamine tetraacetic acid (EDTA) for genetic study and 3ml on a plain tube for thyroid hormones and serum 25-(OH) vitamin D measurement. The blood samples for hormonal assessment were centrifuged then serum samples were stored at --20°C till analysis
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