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| Name | Class |
|---|---|
| Purdue University | OTHER |
| Louisiana State University Health Sciences Center Shreveport | OTHER |
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The purpose of this study is to determine the ability of acute exercise to regulate fat metabolism in muscle of overweight and obese people compared to lean people.
After being informed about the study details and possible risks and benefits, 15 overweight/obese and 15 lean participants gave written informed consent and were then screened and enrolled in the study. All participants then gave a muscle sample and ate a calorically controlled breakfast. Participants completed ~1 hour of cycling exercise on a stationary bike and gave a second muscle sample 4 hours after eating breakfast.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Lean | Active Comparator | males aged 18-30 years with BMI < 25 |
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| Overweight/Obese | Experimental | males aged 18-30 years with BMI >= 25 |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Acute exercise | Behavioral | All participants consumed a standardized, high carbohydrate meal (7 kcal/kg; 60% carbohydrate, 25% fat, 15% protein) and completed an exercise testing trial, expending a total of 650 kcal upon completion 4h later. |
| Measure | Description | Time Frame |
|---|---|---|
| Change from baseline in skeletal muscle acylcarnitines | Metabolomics of skeletal muscle acylcarnitines is used as a measure of mitochondrial function by assessing long, medium and short chain acylcarnitine levels. | Baseline and immediately after acute cycling exercise |
| Change from baseline in skeletal transcriptome | RNA-seq is a method used to quantify changes in all known messenger RNA levels in the genome, giving an assessment of whole genome gene transcription changes. | Baseline and immediately after acute cycling exercise |
| Change from baseline in skeletal muscle whole genome nucleosome maps | MNase-seq is a method used to determine the occupancy/location of nucleosomes throughout the entire genome, supporting evidence of alterations in epigenetic modifications occurring at specific genomic loci that affect nucleosome occupancy/location and possibly gene transcription or silencing. | Baseline and immediately after acute cycling exercise |
| Measure | Description | Time Frame |
|---|---|---|
| Glucose | Commercially validated and available colorimetric used to determine plasma glucose levels. | Baseline and immediately after acute cycling exercise |
| Insulin | Commercially validated and available ELISAs used to determine plasma insulin levels. |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Tara Henagan, PhD | Purdue University, Louisiana State University Medical School at Shreveport | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Purdue University | West Lafayette | Indiana | 47907 | United States |
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| ID | Term |
|---|---|
| D009765 | Obesity |
| D003924 | Diabetes Mellitus, Type 2 |
| D002318 | Cardiovascular Diseases |
| ID | Term |
|---|---|
| D050177 | Overweight |
| D044343 | Overnutrition |
| D009748 | Nutrition Disorders |
| D009750 | Nutritional and Metabolic Diseases |
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| ID | Term |
|---|---|
| D015444 | Exercise |
| ID | Term |
|---|---|
| D009043 | Motor Activity |
| D009068 | Movement |
| D009142 | Musculoskeletal Physiological Phenomena |
| D055687 | Musculoskeletal and Neural Physiological Phenomena |
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All participants underwent the same exact study protocol on random days throughout the study period.
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Investigators conducting molecular analysis were blinded to study samples/groups.
| Baseline and immediately after acute cycling exercise |
| Triglycerides | Commercially validated and available colorimetric kits used to determine plasma triglyceride levels. | Baseline and immediately after acute cycling exercise |
| Free fatty acids | Commercially validated and available colorimetric kits used to determine plasma free fatty acid levels. | Baseline and immediately after acute cycling exercise |
| Cholesterol | Commercially validated and available colorimetric kits used to determine total cholesterol, HDL, LDL and VLDL levels in plasma. | Baseline and immediately after acute cycling exercise |
| D001835 |
| Body Weight |
| D012816 | Signs and Symptoms |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D003920 | Diabetes Mellitus |
| D044882 | Glucose Metabolism Disorders |
| D008659 | Metabolic Diseases |
| D004700 | Endocrine System Diseases |