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The study will assess the exercise-induced changes in chromatin accessibility and gene expression in a cell type-specific manner in skeletal muscle from healthy adults in response to exercise at three different intensities - 1) low-intensity continuous exercise, 2) moderate-intensity continuous exercise, and 3) high-intensity interval exercise.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| low-intensity continuous exercise | Experimental | subjects will exercise at 30% maximal oxygen consumption (VO2max) for 45-60 minutes |
|
| moderate-intensity continuous exercise | Experimental | subjects will exercise at 65% maximal oxygen consumption (VO2max) for 30-45 minutes |
|
| high-intensity interval exercise | Experimental | subjects will completed 10x1minute exercise intervals at maximal oxygen consumption (VO2max) with 1 minute active recovery between |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Low intensity exercise session | Behavioral | subjects will exercise at 30% maximal oxygen consumption (VO2max) for 45-60 minutes |
|
| Measure | Description | Time Frame |
|---|---|---|
| post-translational modifications of skeletal muscle oxidative enzymes | Western blot | 30 minutes before exercise |
| post-translational modifications of skeletal muscle oxidative enzymes | Western blot | immediately after exercise |
| post-translational modifications of skeletal muscle oxidative enzymes | Western blot | 60 minutes after exercise |
| post-translational modifications of Adipose tissue metabolic proteins | Western blot | 15 minutes before exercise |
| post-translational modifications of Adipose tissue metabolic proteins | Western blot | 75 minutes after exercise |
| mRNA expression of skeletal muscle oxidative enzymes | qPCR | 30 minutes before exercise |
| mRNA expression of skeletal muscle oxidative enzymes | qPCR | immediately after exercise |
| mRNA expression of skeletal muscle oxidative enzymes | qPCR | 60 minutes after exercise |
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Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University of Michigan | Ann Arbor | Michigan | 48109 | United States |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 41076654 | Derived | Ahn C, Zhang T, Rode T, Yang G, Chugh OK, Ellis S, Ghayur S, Mehta S, Salzman R, Jiang H, Parker SCJ, Burant CF, Horowitz JF. Molecular responses in abdominal subcutaneous adipose tissue after a session of endurance exercise: effects of exercise intensity. J Physiol. 2025 Nov;603(22):6895-6919. doi: 10.1113/JP289339. Epub 2025 Oct 12. |
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| Moderate intensity exercise session | Behavioral | subjects will exercise at 65% maximal oxygen consumption (VO2max) for 30-45 minutes |
|
| High intensity exercise session | Behavioral | subjects will completed 10x1minute exercise intervals at maximal oxygen consumption (VO2max) with 1 minute active recovery between |
|
| mRNA expression of adipose tissue metabolic enzymes | qPCR | 15 minutes before exercise |
| mRNA expression of adipose tissue metabolic enzymes | qPCR | 75 minutes after exercise |