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Unsuccessful enrollment during COVID-19 pandemic.
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Our objective in this study is to identify the extent to which insulin drives the accumulation of lipids in skeletal muscle of humans. We will test the hypothesis that 4-hours of mild hyperinsulinemia will result in significant muscle lipid accumulation and that such effects will be similar in lean and overweight/obese humans.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Metabolic Study Visit | Experimental | Participants will complete a study visit for metabolic phenotyping and determination of the impact of hyperinsulinemia on outcomes of interest. |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Hyperinsulinemic-euglycemic Clamp | Other | Participants will be administered a constant-rate insulin infusion (to induce mild hyperinsulinemia), with an infusion of dextrose to maintain blood glucose concentration (to maintain euglycemia). |
| Measure | Description | Time Frame |
|---|---|---|
| Changes in skeletal muscle lipid content during hyperinsulinemia compared with basal resting conditions | Liquid chromatography tandem mass spectrometry (targeted lipidomics) will be used to assess species-level changes in skeletal muscle lipid content in biopsy samples collected before and after the insulin infusion to induce mild hyperinsulinemia. | Muscle samples will be collected in basal and hyperinsulinemic conditions separated by ~4.5 hours during the metabolic study visit |
| Measure | Description | Time Frame |
|---|---|---|
| Changes in skeletal muscle mitochondrial oxidative capacity during hyperinsulinemia compared with basal resting conditions | High-resolution respirometry will be used to assess changes in skeletal muscle mitochondrial oxidative capacity in biopsy samples collected before and after the insulin infusion to induce mild hyperinsulinemia. | Muscle samples will be collected in basal and hyperinsulinemic conditions separated by ~4.5 hours during the metabolic study visit |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Sean A Newsom, Ph.D. | Oregon State University | Principal Investigator |
| Matthew M Robinson, Ph.D. | Oregon State University | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Oregon State University | Corvallis | Oregon | 97331 | United States |
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| ID | Term |
|---|---|
| D050177 | Overweight |
| D009765 | Obesity |
| D007333 | Insulin Resistance |
| D008659 | Metabolic Diseases |
| D057185 | Sedentary Behavior |
| ID | Term |
|---|---|
| D044343 | Overnutrition |
| D009748 | Nutrition Disorders |
| D009750 | Nutritional and Metabolic Diseases |
| D001835 | Body Weight |
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Participants will be asked to complete 1 screening visit to determine eligibility.
If participants remain eligible, they will be asked to complete 1 metabolic study visit.
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| D012816 |
| Signs and Symptoms |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D006946 | Hyperinsulinism |
| D044882 | Glucose Metabolism Disorders |
| D001519 | Behavior |