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This study determined the effect of 7 days of high-fat overfeeding on whole-body glycaemic control, glucose kinetics, skeletal muscle insulin signalling, and markers of skeletal muscle microvascular function in 15 healthy young individuals.
Fifteen healthy, young individuals underwent metabolic testing before and after 7 days of high-fat overfeeding. We determined whole-body glycaemic control, glucose kinetics, skeletal muscle insulin signalling, and markers of skeletal muscle microvascular function. Stable isotope tracers were used to assess the rate of appearance of glucose from the gut, liver glucose output and whole-body glucose disposal. Insulin signalling was determined by Western blotting of skeletal muscle tissue. Microvascular function was assessed through the use of quantitative immunofluorescence microscopy. These measurements were performed both postabsorptively and following the ingestion of 50 grams of glucose + 15 grams of protein.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| High-fat diet | Experimental | Participants underwent a 7 day high-fat, high-energy diet intervention with metabolic measurements before and after |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| High-fat, high-energy diet | Dietary Supplement | Participants consumed a diet that was high in fat and high in energy |
|
| Measure | Description | Time Frame |
|---|---|---|
| Change in glucose concentration | Venous blood samples were obtained the day before and the day after the 7 day high-fat diet. Glucose was reported as mmol/L. | Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart) |
| Change in insulin concentration | Venous blood samples were obtained the day before and the day after the 7 day high-fat diet. Insulin was reported as pmol/L. | Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart) |
| Change in glucose kinetics (i.e., rate of appearance and disappearance) assessed using isotope tracers | Glucose kinetics were assessed the day before and the day after the 7 day high-fat diet. Data expressed as umol/kg/min. | Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart) |
| Change in skeletal muscle insulin signalling | AKT and AS160 phosphorylation was determined by Western blotting of skeletal muscle. Data are expressed as arbitrary units. | Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart) |
| Change in markers of microvascular function | endothelial nitric oxide synthase was determined in terminal arterioles by quantitative immunofluorescence microscopy that was performed on sections of skeletal muscle tissue. Data presented as arbitrary units. | Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart) |
| Measure | Description | Time Frame |
|---|---|---|
| Change intramuscular triglyceride content | Intramuscular triglyceride content was determined by quantitative immunofluorescence microscopy that was performed on sections of skeletal muscle tissue. Data presented as arbitrary units. | Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart) |
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Inclusion Criteria:
Exclusion Criteria:
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| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 31513265 | Derived | Parry SA, Turner MC, Woods RM, James LJ, Ferguson RA, Cocks M, Whytock KL, Strauss JA, Shepherd SO, Wagenmakers AJM, van Hall G, Hulston CJ. High-Fat Overfeeding Impairs Peripheral Glucose Metabolism and Muscle Microvascular eNOS Ser1177 Phosphorylation. J Clin Endocrinol Metab. 2020 Jan 1;105(1):dgz018. doi: 10.1210/clinem/dgz018. |
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| ID | Term |
|---|---|
| D007333 | Insulin Resistance |
| ID | Term |
|---|---|
| D006946 | Hyperinsulinism |
| D044882 | Glucose Metabolism Disorders |
| D008659 | Metabolic Diseases |
| D009750 | Nutritional and Metabolic Diseases |
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15 healthy individuals were assessed before and after consuming a high-fat, high-energy diet for 7 days.
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