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| ID | Type | Description | Link |
|---|---|---|---|
| 2020-67001-31214 | Other Grant/Funding Number | NIFA |
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| Name | Class |
|---|---|
| United States Department of Agriculture (USDA) | FED |
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The purpose of this project is to study the molecular and metabolic profiles in human plasma, urine and microbiome composition following the consumption of broccoli sprouts.
The investigators wish to examine metabolic and molecular markers found in blood and in urine after a single dose of sprouts, and evaluate the importance of the gut microbiome on these parameters. Some subjects will receive broccoli sprouts that are grown in deuterium oxide, a commonly used non-toxic, non-radioactive label for vegetables (Tang, Qin et al. 2005), so researchers can identify metabolites in blood and urine that are directly from consumed sprouts. The investigators plan to feed subjects a standardized breakfast and either broccoli sprouts or alfalfa sprouts (control) and look at the presence of metabolites and activity in the blood and urine 3, 6, 24, 48 and 72 hours following intake as well as changes in microbiome composition.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Broccoli sprouts | Active Comparator | Subjects will consume one serving (about 1 cup) of broccoli sprouts with breakfast (bagel, cream cheese, and orange juice). |
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| Deuterium oxide-labeled broccoli sprouts | Active Comparator | Subjects will consume one serving (about 1 cup) of deuterium oxide-labeled broccoli sprouts with breakfast (bagel, cream cheese, and orange juice). |
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| Alfalfa sprouts | Placebo Comparator | Subjects will consume one serving (about 1 cup) of alfalfa sprouts with breakfast (bagel, cream cheese, and orange juice). |
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| Deuterium oxide-labeled alfalfa sprouts | Placebo Comparator | Subjects will consume one serving (about 1 cup) of deuterium oxide-labeled alfalfa sprouts with breakfast (bagel, cream cheese, and orange juice). |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Broccoli sprouts | Other | Subjects will consume one serving (about 1 cup) of broccoli sprouts with breakfast (bagel, cream cheese, and orange juice). |
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| Measure | Description | Time Frame |
|---|---|---|
| Urine sulforaphane | Change in levels of sulforaphane metabolites in urine | Baseline, 3, 6, 24, 48 and 72 hours after consuming a single serving of sprouts |
| Urine sulforaphane metabolites | Change in levels of sulforaphane metabolites in urine | Baseline, 3, 6, 24, 48 and 72 hours after consuming a single serving of sprouts |
| Plasma sulforaphane | Change in levels of sulforaphane in plasma | Baseline, 3, 6, 24, 48 and 72 hours after consuming a single serving of sprouts |
| Plasma sulforaphane metabolites | Change in levels of sulforaphane metabolites in plasma | Baseline, 3, 6, 24, 48 and 72 hours after consuming a single serving of sprouts |
| Stool sample based gut microbiome composition using 16S rRNA gene sequencing | Change in stool sample based gut microbiome composition using 16S rRNA gene sequencing | Baseline, 3, 6, 24, 48 and 72 hours after consuming a single serving of sprouts |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Emily Ho, PhD | Oregon State University | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Oregon State University | Corvallis | Oregon | 97331 | United States |
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participant is blinded to the presence or absence of deuterium label
| Deuterium oxide-labelled broccoli sprouts | Other | Subjects will consume one serving (about 1 cup) of deuterium oxide-labelled broccoli sprouts with breakfast (bagel, cream cheese, and orange juice). |
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| Alfalfa sprouts | Other | Subjects will consume one serving (about 1 cup) of alfalfa sprouts with breakfast (bagel, cream cheese, and orange juice). |
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| Deuterium oxide-labelled alfalfa sprouts | Other | Subjects will consume one serving (about 1 cup) of deuterium oxide-labelled alfalfa sprouts with breakfast (bagel, cream cheese, and orange juice). |
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