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| Name | Class |
|---|---|
| Wellcome Trust | OTHER |
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The present study will investigate the impact of dietary branched chain amino acid restriction on muscle atrophy, insulin sensitivity and muscle protein synthesis following short-term forearm immobilization
Twenty-four healthy young volunteers will undergo 2 days of forearm immobilization in combination with either a branched chain amino acid restricted diet or a control diet. Before and after immobilization, they will receive a stable isotope tracer infusion (5.5 h) combined with repeated blood and muscle sampling under insulin clamp conditions, in order to measure insulin sensitivity and muscle protein synthesis in the fasted and fed state.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Control diet during immobilisation | Placebo Comparator | A 2 day controlled dietary intervention. |
|
| Branched chain amino acid restricted diet during immobilisation | Experimental | A 2 day branched chain amino acid restricted dietary intervention. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Forearm immobilization | Behavioral | Two days of forearm immobilization |
|
| Measure | Description | Time Frame |
|---|---|---|
| Forearm glucose uptake | Insulin sensitivity, measured as forearm glucose uptake, under hyperinsulinaemic-euglycaemic conditions | Hyperinsulinaemic-euglycaemic clamps are performed before and immediately after 2 days of forearm immobilisation. Forearm glucose uptake is measured during the steady state period of the clamp. |
| Measure | Description | Time Frame |
|---|---|---|
| Muscle protein synthesis rates | Muscle protein synthesis rates are measured using an intravenously infused stable isotope labelled amino acid tracer, combined with repeated blood and muscle tissue sampling. | During 3 hours of hyperinsulinaemic-euglycaemic clamped conditions before and after 2 days of immobilisation |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Marlou Dirks, PhD | University of Exeter | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University of Exeter | Exeter | Devon | EX1 2LU | United Kingdom |
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Double blinded
| Intramuscular BCAA concentration |
Intramuscular BCAA concentration measured using mass spectrometry |
| Before and after 3 hours of hyperinsulinaemic-euglycaemic clamped conditions |
| BCAA concentrations between forearm arterialized venous and deep venous blood | Arterialized venous-deep venous forearm balance of BCAA, in the fasted state and during a hyperinsulinaemic-euglycaemic clamp | Fasted and during the steady-state phase of the insulin clamp (i.e. last 30 minutes) |
| Whole-body substrate oxidation | Whole-body substrate oxidation measured using indirect calorimetry | Hyperinsulinaemic-euglycaemic clamps are performed before and immediately after 2 days of forearm immobilisation. Whole-body substrate oxidation is measured before the commencement of, and during the steady state period of the clamp (last 30 minutes) |