The Impact of Leg Immobilization on Postabsorptive and Postprandial Muscle Protein Breakdown in Healthy Young Males

The Impact of Leg Immobilization on Postabsorptive and Postprandial Muscle Protein Breakdown in Healthy Young Males

The present study will seek the quantify the simultaneous muscle protein synthesis and breakdown response with and without amino acid provision in humans following 2 days of immobilisation.

Inclusion Criteria:

• Male
• 18-40 years of age
• Body mass index between 18.5 and 30

Exclusion Criteria:

• Any diagnosed metabolic impairment (e.g. type 1 or 2 Diabetes)
• Any diagnosed cardiovascular disease (e.g. deep vein thrombosis) or hypertension
• Chronic use of any prescribed or over the counter pharmaceuticals (that may modulate muscle protein metabolism)
• A personal or family history of epilepsy, seizures or schizophrenia
• Any known disorders in muscle metabolism
• Regular use of nutritional supplements
• Allergy to lidocaine
• Allergy to one or multiple amino acids
• Recent (within the last 6 months) or current musculoskeletal injury (e.g. leg fracture) as these could be exacerbated by the intervention e.g. unilateral leg immobilisation, or mean the participant is unable to use crutches.
• Having received or ingested a stable isotope tracer in the past