The aim is to assess the impact of physical inactivity on muscle amino acid balance and forearm glucose uptake. In addition, we will evaluate how high-fat overfeeding will modulate muscle amino acid balance and forearm glucose uptake.

The aim is to assess the impact of forearm cast immobilization on muscle amino acid balance and forearm glucose uptake. In addition, we will evaluate how high-fat overfeeding during immobilization will modulate muscle amino acid balance and forearm glucose uptake.

Inclusion Criteria:

• BMI between 18 and 30

Exclusion Criteria:

• Any diagnosed metabolic impairment (e.g. type 1 or 2 Diabetes).
• Any diagnosed cardiovascular disease or hypertension.
• Elevated blood pressure at the time of screening. (An average systolic blood pressure reading of ≥140 mmHg over two or more measurements and an average diastolic blood pressure of ≥90 mmHg over two or more measurements.)
• Chronic use of any prescribed or over the counter pharmaceuticals.
• Regular use of nutritional supplements (e.g. creatine, protein supplementation)
• Metallic implants (including heart pacemaker, cochlear implants, medication pumps, surgical clips, plates or screws).
• A personal or family history of thrombosis, epilepsy, seizures or schizophrenia.
• Any previous motor disorders.