To investigate the short term effect of probiotic oil on plaque acidogenicity among orthodontic patients. Shortage on testing the effect of probiotic on Orthodontic patients can be seen on litterateurs making a gap of whether is product of help in preventing dental caries or not ?

To investigate the short term effect of probiotic oil on plaque acidogenicity among orthodontic patients and to investigate the effect of probiotics on mutan streptococci and lactobacilli in plaque and saliva.

The null-hypothesis is that: The effect of probiotics would not differ from placebo-treated control group.

This product might decrease the chance of getting caries but also cooperation and cost effectiveness should be considered

Inclusion Criteria:

• Subjects undergoing bimaxillary fixed orthodontic treatment
• Number of S. mutans counts (>10.000 CFU/ml)
• Permanent dentition
• 3-12 months into the treatment (study from Sweden by Andrea)
• Medically healthy
• Not taking any medication
• Ability to understand the nature of the study and sign the consent form

Exclusion Criteria:

• Cleft lip and palate patients as well as handicapped and patients with mental impairments
• Systemic diseases or conditions that could interfere with our study (diabetes, immunosuppression diseases, pregnancy or ongoing therapy).
• History of probiotics/anti-inflammatory drugs taken during the last 4 weeks prior to baseline examination
• Local or general antimicrobial substances taken during the last 4 prior weeks prior to baseline examination
• Habitual consumers of xylitol chewing gums
• No active or untreated carious lesions and reported daily tooth brushing habit using fluoridated toothpaste

Both Male and Female matched in age and gender

This research planned for publication

As soon as we finish with the data collection and the manuscript writing, the data will be published online.

3rd party will be involved in labeling the test and placebo samples