This study evaluated the effect of probiotic supplementation on gingival health in orthodontic patients. Participants undergoing fixed orthodontic treatment were randomly assigned to receive either a probiotic preparation containing Limosilactobacillus reuteri or no The primary objective was to assess changes in gingival inflammation using clinical periodontal indices(BOP). Secondary outcomes included plaque accumulation(PI) . The results of this study may help determine whether probiotic supplementation can support gingival health during orthodontic treatment

This was a randomized, controlled clinical trial conducted in orthodontic patients with gingivitis. Participants were allocated to probiotic or no groups and followed for the duration of the orthodontic treatment period. Clinical periodontal parameters were recorded at baseline and at follow-up visits according to the study protocol

Inclusion Criteria:

• Generally healthy patient
• Age 18-59 years
• Fixed-appliance orthodontic treatment
• Plaque-induced gingivitis (BOP > 10%, PD ≤ 4 mm)

Exclusion Criteria:

• Hypersensitivity to product components
• Smoking
• Use of any probiotics within the past 30 days
• Antibiotic or other antimicrobial therapy within the past month
• Use of antibacterial chlorhexidine rinses within the past 2 weeks
• Age < 18 or > 59 years.

De-identified individual participant data (IPD) underlying the results reported in this study, including clinical parameters (bleeding on probing, probing depth, full-mouth plaque index) and microbiological outcomes, will be shared.

Data will be available beginning 6 months after publication and will remain available for 5 years.

Data will be available to researchers who provide a methodologically sound proposal. Proposals should be directed to the corresponding author. Access will be granted after review and approval of the proposal, and a data access agreement may be required.