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A prospective randomized study included 1695 MII sibling oocytes collected from 100 patients undergoing ICSI.
Purpose:
to determine the effect of culture media microdroplet geometry of different culture dishes and oil overlay on the pre implantation embryo development in dry incubators
Method:
We used sibling oocytes splits between two different dishes : GPS (life global, USA) and SPL (life science, Korea) with two different oils : light mineral oil (LM)(Irvine, USA) and paraffin oil (PO)(Vitro life, Sweden). All embryos cultured in dry incubators at the same conditions.
statistical analysis done using SPSS version 23
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| GPS / LM versus SPL/LM | Other | to determine the effect of micro droplet geometry in 3D dishes (GPS) or 2D dishes (SPL) in combination with light mineral oil (LM). |
|
| GPS/PO versus SPL/PO | Other | to determine the effect of micro droplet geometry in 3D dishes (GPS) or 2D dishes (SPL) in combination with paraffin oil (PO). |
|
| GPS/PO versus GPS/LM | Other | to determine the effect of oil overlay light mineral(LM) or paraffin oil (PO) in combination with paraffin oil 3D dishes (GPS). |
|
| SPL/PO versus SPL/LM | Other | to determine the effect of oil overlay light mineral(LM) or paraffin oil (PO) in combination with paraffin oil 2D dishes (SPL). |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| GPS/LM | Other | Media microdroplet geometry (3D dishes) in combination with light mineral oil overlay |
|
| Measure | Description | Time Frame |
|---|---|---|
| Blastulation rate | Percentage of total blastocysts / Total cleaved embryos | 5-6 days after ICSI |
| High quality blast rate | Morphological grading of trophectoderm and Inner cell mass using Gardner's criteria 1999, from grade A and B ( A is the highest quality). Total high grade embryos/ total blast embryos. | 5-6 days after ICSI |
| Measure | Description | Time Frame |
|---|---|---|
| Fertilization rate | Percentage of fertilized oocyte (appearance of two pronuclei) / Number of injected oocytes | 16-19 hours of ICSI |
| Cleavage rate | Percentage of cleaved embryos/ total number of fertilized oocytes |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Manar M Hozyen, MSc. | Ganin Fertility Center | Principal Investigator |
| Amr Elshimy, BSc. | Ganin Fertility Center | Principal Investigator |
| Hanaa Elkhader, BSc. | Ganin Fertility Center | Principal Investigator |
| Hosam Zaki, MSc. | Ganin Fertility Center | Study Chair |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Ganin Fertility Center | Cairo | 11728 | Egypt |
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| ID | Term |
|---|---|
| D007246 | Infertility |
| ID | Term |
|---|---|
| D000091662 | Genital Diseases |
| D000091642 | Urogenital Diseases |
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Effect of two different culture dishes with two different oil overlay which used in human embryos culturing through ICSI procedures on the pre implantaion embryonic development.
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| SPL/LM | Other | Media microdroplet geometry (2D dishes) in combination with light mineral oil overlay |
|
| GPS/PO | Other | Media microdroplet geometry (3D dishes) in combination with Paraffin oil overlay |
|
| SPL/PO | Other | Media microdroplet geometry (2D dishes) in combination with paraffin oil overlay |
|
| 72 hours of ICSI |
| High quality D3 embryos | High quality D3 embryos according to Gardner's criteria / total cleaved embryos | 72 hours of ICSI |
| Good quality trophectoderm/inner cell mass | better grade than 3BB of blast embryos according to Gardner's criteria 1999 | 5-6 days after ICSI |