Culture Media Microdroplet Geometry With Different Oil Overlay and Its Effect on Embryonic Development.

The Effect of Culture Media Microdroplet Geometry and Different Oil Overlay on Pre Implantation Embryonic Development in Dry Incubators: Prospective Randomized Study.

A prospective randomized study included 1695 MII sibling oocytes collected from 100 patients undergoing ICSI.

Purpose:

to determine the effect of culture media microdroplet geometry of different culture dishes and oil overlay on the pre implantation embryo development in dry incubators

Method:

We used sibling oocytes splits between two different dishes : GPS (life global, USA) and SPL (life science, Korea) with two different oils : light mineral oil (LM)(Irvine, USA) and paraffin oil (PO)(Vitro life, Sweden). All embryos cultured in dry incubators at the same conditions.

statistical analysis done using SPSS version 23

Inclusion Criteria:

• Female age is ≤ 37 years old with ≥ 8 MII oocytes
• Males with normal semen parameters (WHO 2010).
• We conducted three experiments all with Siblings oocytes splits and all embryos

Exclusion Criteria:

• Oocyte or sperm donation.
• Sever male factor.
• Any contradictions to undergoing in vitro fertilization or goandotropin stimulation.