Comparison of Human Embryo Development Using Sequential and Single Media With Time-lapse Imaging

Comparison of Human Embryonic Development Using Sequential and Single Media in IVF Monitored by Time-lapse Imaging.

The purpose of this study is to compare human embryo in vitro development in sequential and single-step culture medium using time-lapse imaging.

Objective: To study whether a culture medium that allows undisturbed culture supports human embryo development to the blastocyst stage equivalently to a well-established sequential media.

Design: Randomized, double-blinded sibling trial. Setting: Independent in vitro fertilization (IVF) clinics. Patient(s): One hundred twenty-eight patients, with 1,356 zygotes randomized into two study arms.

Intervention(s): Embryos from each patient were randomly allocated into two study arms to compare embryo development on a time-lapse system using a single-step medium or sequential media.

Inclusion Criteria:

• 6 or more normal embryos following egg retrieval and fertilization.
• Female patients aged 21-40 years or 21-55 years who are using an egg donor.
• Patients using fresh or frozen ejaculated sperm

Exclusion Criteria:

• Previous history of cancer
• Day 3 embryo transfer