Optimizing Platelet Isolation (The Cell Study)

Optimizing Platelet Isolation for Measures of Human Platelet Mitochondrial Respiration (The Cell Study)

The research study is about blood cells (platelets) and their power houses (mitochondria). The investigators want to learn about their behavior in order to ensure that our laboratory procedures are generating optimal results.

The objectives of this study are to assess platelet activation following standard laboratory procedures used in the process of measuring platelet mitochondrial function, and to determine the optimal amount of digitonin needed to permeabilize platelet cell membrane.

Inclusion Criteria:

• Age 25-35
• All races
• All ethnicity
• 18.5 - 30 BMI

Exclusion Criteria:

• smoking
• Aspirin intake
• Recreational drug use
• Alcohol intake more than 3 times per week
• Self-reported possibility of current pregnancy
• Known diagnosis of:
• Pre-diabetes
• Diabetes
• High cholesterol
• High triglycerides
• Hypertension
• Anemia
• Autism spectrum disorder
• Attention deficit hyperactivity disorder (ADHD)
• Attention deficit disorder (ADD)
• Severe asthma
• Refusal to stop taking nutritional supplements
• Any other pre-existing medical condition that requires daily medication as determined by the investigators to affect outcome of interest