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| Name | Class |
|---|---|
| Institut Pasteur de Lille | OTHER |
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It has been reported that Cryptosporidium parvum, a species of a protozoan frequently isolated from humans and animals, is able to induce digestive adenocarcinoma in a rodent model. Consistently, some epidemiological studies have reported an association with cryptosporidiosis in patients with colorectal adenocarcinoma. However, the correlation between cryptosporidiosis and human digestive cancer remains unclear at this time, and it is not known whether this intracellular parasite, considered an opportunistic agent, is able to induce gastrointestinal malignancies in humans. In order to add new arguments for a probable association between cryptosporidiosis and digestive human cancer, the main aim of this study is to determine prevalence and to identify species of Cryptosporidium among a French digestive cancer population.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Patients with colon cancer | Patients with colon cancer of recent diagnosis will be recruited in consultation either in the surgical or gastroenterology departments |
| |
| Patients without colon cancer | Patients without colon cancer but with other gastrointestinal pathology needing a biopsy or a surgical procedure will be recruited either in the surgical or gastroenterology departments |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Detection of Cryptosporidium infection | Diagnostic Test | A biopsy or a surgical sample will be collected from every participant |
|
| Measure | Description | Time Frame |
|---|---|---|
| Prevalence of Cryptosporidium | A biopsy or a surgical sample will be collected from every participant which will be then screened for Cryptosporidium using a Polymerase chain reaction (PCR) in order to obtain a prevalence of positive individuals | 3 years |
| Measure | Description | Time Frame |
|---|---|---|
| Rate of occurrence of parasite development in the tumoral zone (yes/no) compared to the peri-tumoral zone. | A biopsy or a surgical sample containing the tumoral and peri-tumoral areas will be collected only from the cases which will be then screened for Cryptosporidium using PCR | 3 years |
| Significance of the association between the histological grade of the lesion and the Cryptosporidium species |
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Inclusion Criteria:
Cases:
Controls:
Exclusion Criteria:
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The cases will be the patients with colon cancer of recent diagnosis recruited in consultation either in the surgical or gastroenterology departments. The controls will be the patients without colon cancer but with other gastrointestinal pathology needing a biopsy or a surgical procedure and recruited either in the surgical or gastroenterology departments
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Marie Paule Lebitasy, MD | Contact | 0033320225269 | Lebitasy.Marie-Paule@ghicl.net | |
| Berenice Marchant | Contact | 0033320225751 | marchant.berenice@ghicl.net |
| Name | Affiliation | Role |
|---|---|---|
| Bertrand Nunes, MD | GHICL | Study Director |
| Gabriela Certad, MD, PhD | GHICL | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Groupement des Hôpitaux de l'Institut Catholique de Lille | Recruiting | Lomme | Nord | 59462 | France |
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| ID | Term |
|---|---|
| D003110 | Colonic Neoplasms |
| D003457 | Cryptosporidiosis |
| D007239 | Infections |
| D009369 | Neoplasms |
| ID | Term |
|---|---|
| D015179 | Colorectal Neoplasms |
| D007414 | Intestinal Neoplasms |
| D005770 | Gastrointestinal Neoplasms |
| D004067 | Digestive System Neoplasms |
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Surgical specimens or biopsies
Significance of the association between the histological grade defined by the Vienna classification of tumors and the species of Cryptosporidium and the Cryptosporidium species.The main species of Cryptosporidium infecting humans are C. parvum and C. hominis, however, other species can also infect humans. |
| 3 years |
| Significance of the association between the histological grade of the lesion and the parasitic charge in tissues | Significance of the association between the histological grade defined by the Vienna classification of tumors and the species of Cryptosporidium and the parasitic charge in the tissues that will be determined by quantitative polymerase chain reaction (qPCR) | 3 years |
| The rate of change in the expression or localization of certain markers known or suspected to be involved in the process of carcinogenesis. | Some markers known to be associated to cancer development will be explored such as: Beta-catenin, P53, APC (adenomatous polyposis coli), etc. | 3years |
| The association between the rate of lymphocytes (CD3, CD4, CD8, CD19 and CD4/CD8 ratio) and the presence of parasites in biopsies or surgical specimens | Because Cryptosporidium is an opportunistic agent that causes significant morbidity and mortality in immunocompromised patients, it is possible that individuals with malignancies, have a higher risk of developing infection with this parasite, especially when their immunosuppression is more severe. To rule out this possibility this association will be determined. | 3 years |
| Rate of development of a neoplastic lesion in the human explants | A three-dimensional culture model will be developed using colon samples from healthy tissues of several control patients. An experimental Cryptosporidium infection will be tested in order to determine the development of neoplastic lesions in these human explants. | 3 years |
| D009371 | Neoplasms by Site |
| D004066 | Digestive System Diseases |
| D005767 | Gastrointestinal Diseases |
| D003108 | Colonic Diseases |
| D007410 | Intestinal Diseases |
| D007411 | Intestinal Diseases, Parasitic |
| D010272 | Parasitic Diseases |
| D011529 | Protozoan Infections, Animal |
| D010273 | Parasitic Diseases, Animal |
| D003048 | Coccidiosis |
| D011528 | Protozoan Infections |
| D000820 | Animal Diseases |