Colorectal cancer (CRC) is reported to be the third leading cause of cancer deaths worldwide, and its prevalence is steadily rising in developing countries. It can be considered a marker of socioeconomic development and a marker of significant changes in food habits and lifestyle. In Egypt, CRC represents the seventh most common cancer. It is the third most reported cancer in males and the fifth most common cancer in females. microbiome dysbiosis may be involved in the pathogensis and thus pose a therapeutic target

Many reports suggested the role of westernized dietary lifestyles, including high consumption of red meats, animal fats, and cholesterol-rich foods, as well as smoking, decreased physical activity, obesity, diabetes, alcohol consumption, or smoking, in CRC development. These changes in lifestyle are called the external factors, which are now linked to changes in the inner gut microenvironments, mainly the microbiomes. Our aim is to investigate the the changes in microbiome profile in relation to clinical, pathological and lifestyle factors

Inclusion Criteria:

• Age of 40 years to 80 years
• Patients with a newly diagnosed CRC

Exclusion Criteria:

• Patients with previously known CRC
• Pregnancy or Lactation
• Abdominal surgery within/up to 6 months prior to sample collection
• Antibiotics, corticosteroids, or probiotics up to three months prior to sample collection
• History of other types of cancer
• Subjects on a special diet
• Diabetes, liver, or kidney diseases
• CRC patients who used enema or underwent colonoscopy up to one week before sample collection.

12 newly diagnosed CRC patiens vs 6 healthy voulnteers

Patients' information will be assigned codes. no need to reveal it