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The aim of this research is to evaluate thiol-disulfide balance in cervix cancer patients
To date various oxidative stress parameters were studied in patients with cervix cancer, however dynamic thiol disulfide hemostasis has not been previously investigated.
The thiol-disulfide balance in the circulation will be measured by the automated system created by Erel & Neselioglu (1).
References
1- Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem 2014;47:326-32. doi:10.1016/j.clinbiochem.2014.09.026.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Cervix cancer | Cervix cancer n=62 |
| |
| Healthy controls | Healthy volunteers n=61 |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Disulphide(μmol/L) | Diagnostic Test | Aforementioned oxidative stress markers will be measured via Cobas c501(Roche Diagnostics, Indianapolis, IN, USA) used with spectrophotometry |
|
| Measure | Description | Time Frame |
|---|---|---|
| serum native thiol level | serum native thiol level in μmol/L | preoperative in the morning or preprandial for healthy volunteers |
| serum disulphide level | serum disulphide level in μmol/L | preoperative in the morning or preprandial for healthy volunteers |
| serum total thiol level | serum total thiol level in μmol/L | preoperative in the morning or preprandial for healthy volunteers |
| the disulphide/native thiol ratio | the disulphide/native thiol ratio X (100 ) | preoperative in the morning or preprandial for healthy volunteers |
| disulphide/total thiol ratio | disulphide/total thiol ratio X (100 ) | preoperative in the morning or preprandial for healthy volunteers |
| native thiol/total thiol ratio | native thiol/total thiol ratio X (100 ) | preoperative in the morning or preprandial for healthy volunteers |
| Measure | Description | Time Frame |
|---|---|---|
| total oxidant capacity (TOC) | TOC in mmol H2O2 equivalent/L | preoperative in the morning or preprandial for healthy volunteers |
| total antioxidant capacity (TAC) | TAC in mmol trolox equivalent/L |
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Inclusion Criteria:
• Histopathologically confirmed diagnosis of cervix cancer
Exclusion Criteria:
cervix cancer
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The preoperative diagnosis of cervix cancer was made histopathologically by cervical biopsy. An experienced pathologist performed pathological analysis according to the surgical staging scheme of the International Federation of Gynaecology and Obstetrics.
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| Name | Affiliation | Role |
|---|---|---|
| Burak Sezgin, MD | Mugla Sıtkı Kocman University Faculty of Medicine | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Mugla Sıtkı Kocman University Faculty of Medicine | Muğla | 48000 | Turkey (Türkiye) |
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| ID | Term |
|---|---|
| D002583 | Uterine Cervical Neoplasms |
| D004194 | Disease |
| ID | Term |
|---|---|
| D014594 | Uterine Neoplasms |
| D005833 | Genital Neoplasms, Female |
| D014565 | Urogenital Neoplasms |
| D009371 | Neoplasms by Site |
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serum blood samples for each participant
|
| Total Oxidant Capacity(μmolH2O2Equiv/L) | Diagnostic Test | Aforementioned oxidative stress markers will be measured via Cobas c501(Roche Diagnostics, Indianapolis, IN, USA) used with spectrophotometry |
|
|
| Ischemia Modified Albumin(IU/mL) | Diagnostic Test | The serum Ischemia Modified Albumin concentrations were measured as described by Bar-Or et al. (Bar-Or et al. 2000). |
|
| preoperative in the morning or preprandial for healthy volunteers |
| Oxidative stress index (OSI) | OSI in mmol trolox/L. | preoperative in the morning or preprandial for healthy volunteers |
| ischaemia-modified albumin | ischaemia-modified albumin in IU/mL | preoperative in the morning or preprandial for healthy volunteers |
| D009369 |
| Neoplasms |
| D002577 | Uterine Cervical Diseases |
| D014591 | Uterine Diseases |
| D005831 | Genital Diseases, Female |
| D052776 | Female Urogenital Diseases |
| D005261 | Female Urogenital Diseases and Pregnancy Complications |
| D000091642 | Urogenital Diseases |
| D000091662 | Genital Diseases |
| D010335 | Pathologic Processes |
| D013568 | Pathological Conditions, Signs and Symptoms |